Bonte W, Bleifuss J, Volck J
Forensic Sci. 1976 Jan-Feb;7(1):9-22. doi: 10.1016/0300-9432(76)90003-0.
Samples of thoracic fluid were obtained at regular intervals from the putrefying bodies of dead dogs. For quantitative estimation of the proteins two-dimensional immunoelectrophoresis was employed and for the amino acids and amines two-dimensional thin-layer chromatography. The results lead to speculation that the initially lagging proteolytic changes are due to catalysis of autoenzymes, whereas bacteriological processes are responsible for a distinct acceleration from the 20th day after death. At this time the amino-acid concentrations showed a marked increase, which is followed by a maximum of bacterial growth, that ultimately leads to a sudden regression of amino acids. Simultaneously amines appear. The drying up of the thoracic cavity after 35 to 45 days marks the change from liquefaction to the terminal decay.
从死亡犬类的腐败尸体上定期采集胸水样本。对于蛋白质的定量测定，采用二维免疫电泳法；对于氨基酸和胺类，采用二维薄层色谱法。结果表明，最初滞后的蛋白水解变化是由于自身酶的催化作用，而细菌学过程则导致死后第20天起出现明显加速。此时氨基酸浓度显著增加，随后细菌生长达到最大值，最终导致氨基酸突然减少。同时出现胺类。35至45天后胸腔干涸，标志着从液化到末期腐烂的转变。
