Paul W E, Thorbecke G J, Siskind G W, Benacerraf B
Immunology. 1969 Jul;17(1):85-92.
The amount of antibody produced by BSA-tolerant rabbits as a result of immunization with DNP—BSA is dependent upon the amount of BSA used to induce tolerance. Tolerance was induced by initial injection of 100 μg of antigen followed by progressively higher doses. Rabbits rendered tolerant with a maximum BSA dose of 1 mg had a mean serum anti-BSA antibody concentration of 0.39 mg/ml after immunization with DNP—BSA, whereas rabbits rendered tolerant with a maximum BSA dose of 100 mg had a mean serum anti-BSA concentration of 0.02 mg after the same course of immunization. This compares with a mean of 1.08 mg of anti-BSA antibody in normal rabbits immunized with DNP—BSA. There was a similar reduction in the concentration of anti-DNP antibodies and of conjugate-specific antibodies in the tolerant groups. The results are discussed in terms of a thermodynamically-controlled induction of tolerance in individual precursor cells.
用二硝基苯牛血清白蛋白（DNP-BSA）免疫对牛血清白蛋白（BSA）耐受的兔子所产生的抗体量，取决于用于诱导耐受的BSA量。通过先注射100μg抗原，随后逐步增加剂量来诱导耐受。用最大剂量为1mg BSA诱导耐受的兔子，在用DNP-BSA免疫后，血清抗BSA抗体平均浓度为0.39mg/ml，而用最大剂量为100mg BSA诱导耐受的兔子，在相同免疫过程后，血清抗BSA平均浓度为0.02mg。相比之下，用DNP-BSA免疫的正常兔子中，抗BSA抗体平均为1.08mg。耐受组中抗DNP抗体和结合物特异性抗体的浓度也有类似降低。根据单个前体细胞中耐受性的热力学控制诱导来讨论这些结果。
