Simon W H, Richardson S, Herman W, Parsons J R, Lane J
J Bone Joint Surg Am. 1976 Jun;58(4):517-26.
The effects of freezing localized areas of rabbit articular cartilage in vivo were studied at two to twelve months morphologically (gross and microscopic examination, including scanning electron microscopy), histochemically (toluidine blue and safranin O), and metabolically (35S uptake). Up to six months the frozen cartilage was intact but appeared to be dead, as shown by the absence of stainable chondrocytes, severely decreased acid mucopolysaccharide content, and absence of significant uptake of 35S. At twelve months fibrillation and softening were evident, clusters of new chondrocytes with surrounding acid mucopolysaccharides were visible microscopically, and scanning electron microscopy revealed an irregular pattern of collagen fibers which were larger than normal, of varying thickness, and broken in some areas. These changes resembled those seen in degenerative joint disease.
对兔关节软骨局部区域进行体内冷冻，在两到十二个月的时间里，从形态学（大体和显微镜检查，包括扫描电子显微镜）、组织化学（甲苯胺蓝和番红O）和代谢（35S摄取）方面进行了研究。在长达六个月的时间里，冷冻软骨保持完整，但似乎已经死亡，表现为无可染色的软骨细胞、酸性粘多糖含量严重降低以及35S摄取量显著缺乏。在十二个月时，明显出现了纤维化和软化，显微镜下可见成群的新软骨细胞及其周围的酸性粘多糖，扫描电子显微镜显示胶原纤维呈不规则形态，比正常的更大，厚度各异，且在某些区域断裂。这些变化类似于退行性关节病中所见的变化。
