Bjelle A
Scand J Rheumatol. 1977;6(1):37-44.
Biopsy specimens of human articular cartilage were taken from the caudal summits and from the posterior part of both epicondyles of the right lower femoral epiphysis of 20 individuals within 12 hours post mortem. 12 articular surfaces were normal and 8 included changes attributable to osteoarthrosis. In one individual with osteoarthrotic changes the articular cartilage surface was subjected to a more detailed study. CPC- and ECTEOLA-cellulose column chromatography of glycosaminoglycans was performed and the content and collagen contents were determined. A reduced ratio of chondroitin to keratan sulphates in articular cartilage was found to be related to age. In osteoarthrosis this change was more pronounced and both chondroitin sulphate and keratan sulphate were of lower molecular weight and/or sulphate content. On approaching eburnized cartilage surfaces, decreasing concentrations of glycosaminoglycans were found, concomitant with a corresponding increase in non-collagenous proteins. No change in hyaluronic acid concentration was found with advancing age or in osteoarthrosis.
在死后12小时内，从20名个体右下肢股骨骨骺的尾端以及两个髁的后部获取人关节软骨活检标本。12个关节表面正常，8个存在骨关节炎所致的改变。对一名有关节炎改变的个体的关节软骨表面进行了更详细的研究。对糖胺聚糖进行了CPC和ECTEOLA - 纤维素柱色谱分析，并测定了其含量和胶原蛋白含量。发现关节软骨中硫酸软骨素与硫酸角质素的比例降低与年龄有关。在骨关节炎中，这种变化更为明显，硫酸软骨素和硫酸角质素的分子量和/或硫酸盐含量均较低。在接近象牙样变的软骨表面时，发现糖胺聚糖浓度降低，同时非胶原蛋白相应增加。随着年龄增长或在骨关节炎中，透明质酸浓度未发现变化。
