Gelbke H P, Schlicht H J, Schmidt G
Arch Toxicol. 1977 Nov 21;38(4):295-305. doi: 10.1007/BF00352034.
A radioimmunoassay for the determination of diazepam in human blood and serum is presented. Diazepam is separated from the bulk of the biological material by adsorption at Amberlite XAD-2 and subsequent desorption with ethyl acetate. The extract thus obtained can be used directly for the determination od diazepam by radioimmunoassay and gas chromatography with electron capture detection. For the combined radioimmunological and gas chromatographic determination 0.5 ml of blood or serum are necessary, the lower detection limit being approx. 5 ng/ml for both of these procedures. A good reliability of the radioimmunoassay is established with respect to precision, accuracy and specificity. The quantitative results obtained by radioimmunoassay and gas chromatography correspond well with each other. The radioimmunological procedure enables the screening of more than 200 samples for diazepam by one technician within two working days.
本文介绍了一种用于测定人血液和血清中地西泮的放射免疫分析法。地西泮通过在Amberlite XAD - 2上吸附并随后用乙酸乙酯解吸，与大部分生物材料分离。由此获得的提取物可直接用于通过放射免疫分析法和带有电子捕获检测的气相色谱法测定地西泮。对于放射免疫和气相色谱联合测定，需要0.5毫升血液或血清，这两种方法的检测下限约为5纳克/毫升。在精密度、准确度和特异性方面，放射免疫分析法具有良好的可靠性。通过放射免疫分析法和气相色谱法获得的定量结果彼此吻合良好。放射免疫程序使一名技术人员能够在两个工作日内筛选200多个样品中的地西泮。
