Isakson P C, Puré E, Uhr J W, Vitetta E S
Proc Natl Acad Sci U S A. 1981 Apr;78(4):2507-11. doi: 10.1073/pnas.78.4.2507.
Sepharose-bound anti-immunoglobulins, which are potent mitogens for normal adult B cells, are not mitogenic for tumor cells freshly isolated from mice carrying the B-cell leukemia BCL1. However, after 4 or more days of in vitro cultivation, BCL1 cells can be stimulated to divide by either anti-mu or anti-delta antibodies. These results suggest that in vitro cultivation of BCL1 cells results in their differentiation into more mature cells which can be triggered to proliferate by their interaction with anti-Ig antibodies. Addition of T-cell helper factors to anti-Ia treated BCL1 cells results in their differentiation into Ig-secreting cells. These results indicate that surface Ig molecules on BCL1 cells are capable of delivering an activation signal to the cells but that the cells require a second signal from T cells for induction of Ig secretion.
与琼脂糖珠结合的抗免疫球蛋白，对正常成年B细胞是有效的促有丝分裂原，但对从携带B细胞白血病BCL1的小鼠新鲜分离的肿瘤细胞没有促有丝分裂作用。然而，在体外培养4天或更长时间后，抗μ或抗δ抗体可刺激BCL1细胞分裂。这些结果表明，BCL1细胞的体外培养导致其分化为更成熟的细胞，这些细胞可通过与抗Ig抗体相互作用而被触发增殖。向抗Ia处理的BCL1细胞中添加T细胞辅助因子会导致其分化为分泌Ig的细胞。这些结果表明，BCL1细胞表面的Ig分子能够向细胞传递激活信号，但细胞需要来自T细胞的第二个信号来诱导Ig分泌。
