Ahlborg U G, Thunberg T
Arch Toxicol. 1978 Feb 21;40(1):55-61. doi: 10.1007/BF00353279.
The metabolism of pentachlorophenol has been studied in the rat after pretreatments with phenobarbital, 3-methyl cholanthrene or 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). In addition to the previously identified metabolite, tetrachloro-p-hydroquinone, trichloro-p-hydroquinone has been identified in urine as a metabolite. The formation of the latter represents a type dechlorination different from that of the formation of tetrachlorohydroquinone. The inducing agents, 3-methylcholanthrene and TCDD have similar effects on the dechlorination and increase the formation of tetrachloro-p-hydroquinone more pronounced than does phenobarbital. In contrast to phenobarbital they also increase the formation of trichloro-p-hydroquinone and the total elimination of pentachlorophenol and its metabolites. The in vivo findings are supported by in vitro studies with microsomes from rats pretreated with phenobarbital or TCDD. Use of the inhibitor beta-diethylaminoethyl-diphenyl propylacetate (SKF 525-A) in vitro showed a more pronounced inhibition on microsomes from phenobarbital-treated rats than on microsomes from untreated or TCDD-treated rats. Gas chromatography-mass spectrometry have been used for the identification and quantification of pentachlorophenol and its metabolites.
在大鼠中研究了用苯巴比妥、3-甲基胆蒽或2,3,7,8-四氯二苯并对二噁英（TCDD）预处理后五氯苯酚的代谢情况。除了先前鉴定出的代谢产物四氯对苯二酚外，尿中还鉴定出三氯对苯二酚为代谢产物。后者的形成代表了一种与四氯对苯二酚形成不同类型的脱氯作用。诱导剂3-甲基胆蒽和TCDD对脱氯作用有相似的影响，且比苯巴比妥更显著地增加四氯对苯二酚的形成。与苯巴比妥不同的是，它们还增加三氯对苯二酚的形成以及五氯苯酚及其代谢产物的总消除量。体内研究结果得到了用苯巴比妥或TCDD预处理的大鼠微粒体的体外研究的支持。体外使用抑制剂β-二乙氨基乙基二苯基丙基乙酸酯（SKF 525-A）显示，对苯巴比妥处理的大鼠微粒体的抑制作用比对未处理或TCDD处理的大鼠微粒体更显著。气相色谱-质谱法已用于五氯苯酚及其代谢产物的鉴定和定量。
