氘代磷脂作为生物膜拉曼研究中的非干扰成分。
Deuterated phospholipids as nonperturbing components for Raman studies of biomembranes.
作者信息
Gaber B P, Yager P, Peticolas W L
出版信息
Biophys J. 1978 May;22(2):191-207. doi: 10.1016/S0006-3495(78)85484-8.
Abstract
The deuterated phospholipid, 1,2-dipalmitoyl-d62-phosphatidylcholine is shown by Raman spectroscopic measurements to be useful for obtaining information concerning phospholipid conformation in complex phospholipid and lipidprotein mixtures. The Raman bands of the deuterated phospholipid are assigned, and the sensitivity of these vibrational modes to conformational changes in the bilayer is demonstrated. Deuteration of the alkyl chains reveals the CH vibrations of the head group. A change in these bands is observed at the melting temperature and is assigned to alteration of the glycerol backbone conformation upon melting.
摘要
氘代磷脂1,2-二棕榈酰-d62-磷脂酰胆碱经拉曼光谱测量表明，可用于获取有关复杂磷脂和脂蛋白混合物中磷脂构象的信息。对氘代磷脂的拉曼谱带进行了归属，并证明了这些振动模式对双层构象变化的敏感性。烷基链的氘代揭示了头部基团的CH振动。在熔点温度下观察到这些谱带的变化，并将其归因于熔化时甘油主链构象的改变。
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