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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Biochem J. 1969 May;112(5):721-7. doi: 10.1042/bj1120721.

The conditions affecting the activity of RNA polymerase in isolated rat liver nuclei were studied with Mg(2+) or Mn(2+) as activating ions. 2. The enzyme assayed with Mg(2+) and at low ionic strength is saturated by a lower concentration of nucleotide substrates than if assayed with Mn(2+) at low ionic strength or with either ion at high ionic strength. 3. At low and at high ionic strength the incorporation of AMP is affected in a similar way by variations in the temperature of incubation. Preincubation at 37 degrees impairs the AMP incorporation. 4. Heparin stimulates the RNA polymerase activity in the presence of Mn(2+). 5. Both ammonium sulphate and heparin ;restart' the reaction if added after 15min., the effect being more marked with ammonium sulphate than with heparin, and also more marked in the presence of Mn(2+) than of Mg(2+). 6. alpha-Amanitin abolishes the effect of ammonium sulphate and of heparin.

以Mg(2+)或Mn(2+)作为激活离子，研究了影响离体大鼠肝细胞核中RNA聚合酶活性的条件。2. 在低离子强度下用Mg(2+)测定的酶，比在低离子强度下用Mn(2+)测定或在高离子强度下用任何一种离子测定时，被较低浓度的核苷酸底物饱和。3. 在低离子强度和高离子强度下，孵育温度的变化对AMP掺入的影响方式相似。在37℃预孵育会损害AMP掺入。4. 在Mn(2+)存在下，肝素刺激RNA聚合酶活性。5. 如果在15分钟后添加，硫酸铵和肝素都能“重启”反应，硫酸铵的效果比肝素更明显，并且在Mn(2+)存在下比在Mg(2+)存在下更明显。6. α-鹅膏蕈碱消除了硫酸铵和肝素的作用。

Biochem J. 1969 May;112(5):721-7. doi: 10.1042/bj1120721.

The conditions affecting the activity of RNA polymerase in isolated rat liver nuclei were studied with Mg(2+) or Mn(2+) as activating ions. 2. The enzyme assayed with Mg(2+) and at low ionic strength is saturated by a lower concentration of nucleotide substrates than if assayed with Mn(2+) at low ionic strength or with either ion at high ionic strength. 3. At low and at high ionic strength the incorporation of AMP is affected in a similar way by variations in the temperature of incubation. Preincubation at 37 degrees impairs the AMP incorporation. 4. Heparin stimulates the RNA polymerase activity in the presence of Mn(2+). 5. Both ammonium sulphate and heparin ;restart' the reaction if added after 15min., the effect being more marked with ammonium sulphate than with heparin, and also more marked in the presence of Mn(2+) than of Mg(2+). 6. alpha-Amanitin abolishes the effect of ammonium sulphate and of heparin.

以Mg(2+)或Mn(2+)作为激活离子，研究了影响离体大鼠肝细胞核中RNA聚合酶活性的条件。2. 在低离子强度下用Mg(2+)测定的酶，比在低离子强度下用Mn(2+)测定或在高离子强度下用任何一种离子测定时，被较低浓度的核苷酸底物饱和。3. 在低离子强度和高离子强度下，孵育温度的变化对AMP掺入的影响方式相似。在37℃预孵育会损害AMP掺入。4. 在Mn(2+)存在下，肝素刺激RNA聚合酶活性。5. 如果在15分钟后添加，硫酸铵和肝素都能“重启”反应，硫酸铵的效果比肝素更明显，并且在Mn(2+)存在下比在Mg(2+)存在下更明显。6. α-鹅膏蕈碱消除了硫酸铵和肝素的作用。