Woelk H
Arzneimittelforschung. 1979;29(4):615-7.
In search of the biochemical mechanism of the action of 2-oxopyrrolidine-1-acetamide (piracetam, Normabrain, Nootrop) the influence of the encephalotropic substance on the hydrolysis of different glycerophosphatides by the neuronal and synaptosomal phospholipase A2 was investigated. Pure synaptosomes and neurons were prepared by a new method. The synaptosomal and neuronal phospholipase A2 plays an important role during the process of synaptic transmission. The administration of piracetam enhanced both the neuronal and synaptosomal phospholipase A2-activity by about 50%. The piracetam-induced stimulation of the enzyme activity was in the same order of magnitude for all substrates investigated. Thus piracetam stimulates the synaptic transmission. The stimulation of the phospholipase A2-activity by piracetam appears to be mediated by a neurotransmitter.
为探寻2-氧代吡咯烷-1-乙酰胺（吡拉西坦，脑复康，Nootrop）的作用生化机制，研究了这种促脑物质对神经元及突触体磷脂酶A2水解不同甘油磷脂的影响。采用一种新方法制备了纯突触体和神经元。突触体及神经元磷脂酶A2在突触传递过程中起重要作用。给予吡拉西坦可使神经元及突触体磷脂酶A2活性均增强约50%。对于所有研究的底物，吡拉西坦诱导的酶活性刺激程度处于同一数量级。因此，吡拉西坦可刺激突触传递。吡拉西坦对磷脂酶A2活性的刺激似乎是由一种神经递质介导的。
