用亲和层析法纯化银环蛇抗蛇毒血清

Zhonghua Min Guo Wei Sheng Wu Xue Za Zhi. 1978 Dec;11(4):122-9.

Bungarus multicinctus horse antivenin was purified by ammonium sulfate fractionation, gel filtration on Sephadex G-200 column, and affinity chromatography. The specific neutralizing capacity of the antibody preparations showed 2.21-fold, 3.12-fold, and 29.6-fold higher than that of the crude antivenin, respectively.

银环蛇马抗蛇毒血清经硫酸铵分级分离、Sephadex G - 200柱凝胶过滤和亲和层析进行纯化。抗体制剂的特异性中和能力分别比粗制抗蛇毒血清高2.21倍、3.12倍和29.6倍。