尿囊链球菌的脲基乙醇酸合成酶。I. 乙醛酸的测定及酶的纯化。
Ureidoglycolate synthetase of Streptococcus allantoicus. I. Measurement of glyoxylate and enzyme purification.
作者信息
Gaudy E T, Bojanowski R, Valentine R C, Wolfe R S
出版信息
J Bacteriol. 1965 Dec;90(6):1525-30. doi: 10.1128/jb.90.6.1525-1530.1965.
Gaudy, Elizabeth T. (University of Illinois, Urbana), R. Bojanowski, R. C. Valentine, and R. S. Wolfe. Ureidoglycolate synthetase of Streptococcus allantoicus. I. Measurement of glyoxylate and enzyme purification. J. Bacteriol. 90:1525-1530. 1965.-A new spectrophotometric method for the determination of glyoxylate is described. The technique is based on measurement of the initial rate of formation of glyoxylic acid phenylhydrazone in neutral solution. Its advantages include rapidity and convenience, suitability for use with mixtures containing acid-labile substrates, and elimination of possibly inhibitory reagents from the enzyme incubation mixture. Ureidoglycolate synthetase, which cleaves ureidoglycolate to glyoxylate and urea, was purified from crude extracts of Streptococcus allantoicus grown on allantoin-containing medium. The purification procedures include treatment with MnCl(2), fractionation on calcium phosphate gel, fractional precipitation with ammonium sulfate, and column chromatography on diethylaminoethyl cellulose. The final enzyme preparation was purified 77-fold and contained 35% of the total activity of the extract.
高迪，伊丽莎白·T.（伊利诺伊大学厄巴纳分校），R.博亚诺夫斯基，R.C.瓦伦丁，以及R.S.沃尔夫。尿囊链球菌的脲基乙醇酸合成酶。I.乙醛酸的测定及酶的纯化。《细菌学杂志》90:1525 - 1530。1965年。——描述了一种测定乙醛酸的新分光光度法。该技术基于在中性溶液中测定乙醛酸苯腙形成的初始速率。其优点包括快速、方便，适用于含有酸不稳定底物的混合物，以及从酶孵育混合物中去除可能具有抑制作用的试剂。脲基乙醇酸合成酶可将脲基乙醇酸裂解为乙醛酸和尿素，它是从在含尿囊素培养基上生长的尿囊链球菌粗提物中纯化得到的。纯化步骤包括用氯化锰处理、在磷酸钙凝胶上分级分离、用硫酸铵分级沉淀以及在二乙氨基乙基纤维素上进行柱层析。最终的酶制剂纯化了77倍，含有提取物总活性的35%。
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