Tian Haibing, Xie Wanyi, He Shixuan, Zhou Daming, Fang Shaoxi, Liang Liyuan, Wang Deqiang
Chongqing Institute of Green and Intelligent Technology, Chinese Academy of Science Chongqing 400714 P. R. China
University of Chinese Academy of Sciences P. R. China.
RSC Adv. 2019 May 17;9(27):15431-15436. doi: 10.1039/c9ra00698b. eCollection 2019 May 14.
Nanopore technology was introduced for the study of the dynamic interactions between bovine serum albumin (BSA) and 1,2-dioleoyl--glycero-3-phosphoethanolamine (DOPE) phospholipids based on a modified nanopore. The results reveal that the interaction mechanism between DOPE and BSA is affected by the pH of the subphase. Far above the BSA isoelectric point (pH > 7), a weaker hydrophobic interaction and stronger electrostatic repulsion exist between the DOPE and BSA molecules. At pH = 7, the BSA structure nearly does not change, and the interaction is weak. At pH 5 and pH 6, BSA is marginally affected by the adsorption interaction, and below pH 5, the DOPE film becomes disordered, so there is a strong repulsive force interaction between the BSA and DOPE.
基于一种改进的纳米孔，引入了纳米孔技术来研究牛血清白蛋白（BSA）与1,2-二油酰基-sn-甘油-3-磷酸乙醇胺（DOPE）磷脂之间的动态相互作用。结果表明，DOPE与BSA之间的相互作用机制受亚相pH值的影响。在远高于BSA等电点（pH > 7）时，DOPE与BSA分子之间存在较弱的疏水相互作用和较强的静电排斥作用。在pH = 7时，BSA结构几乎不变，相互作用较弱。在pH 5和pH 6时，BSA受到吸附相互作用的影响较小，而在pH 5以下，DOPE膜变得无序，因此BSA与DOPE之间存在强烈的排斥力相互作用。
