Beidler L M, Smallman R L
J Cell Biol. 1965 Nov;27(2):263-72. doi: 10.1083/jcb.27.2.263.
Colchicine blocks mitotic division of the epithelial cells surrounding the taste bud of the rat tongue. Response to chemical stimulation decreases 50 per cent 3 hours after colchicine injection as measured by recording the electrical activity from the taste nerve bundle. Radioautography, using tritiated thymidine, shows that those epithelial cells surrounding the taste bud divide and that some of the daughter cells enter the taste bud and slowly move toward the center. The life span of the average cell is about 250 +/- 50 hours, although some cells have a much shorter and others a much longer life span. These studies suggest that the cells within the taste bud, as well as the nerves, undergo considerable change with time. Corresponding changes in function are considered.
秋水仙碱可阻断大鼠舌味蕾周围上皮细胞的有丝分裂。注射秋水仙碱3小时后，通过记录味觉神经束的电活动来测量，对化学刺激的反应降低了50%。使用氚标记胸腺嘧啶核苷进行放射自显影显示，味蕾周围的那些上皮细胞会分裂，并且一些子细胞进入味蕾并缓慢向中心移动。尽管有些细胞的寿命短得多，而另一些则长得多，但平均细胞寿命约为250±50小时。这些研究表明，味蕾内的细胞以及神经会随着时间发生相当大的变化。同时考虑了相应的功能变化。
