Zakin M M, Peña C, Poskus E, Stewart J M, Paladini A C
Eur J Immunol. 1977 Oct;7(10):701-4. doi: 10.1002/eji.1830071010.
The immunological properties of a synthetic human growth hormone fragment comprising the amino acids 73 through 128 and of the homologous natural horse growth fragment formed by amino acids 73 through 123, have been comparatively studied. Antisera obtained in rabbits inoculated with the native human hormone or with the fragments, were used. By hemagglutination experiments both fragments have the same reactivity toward the anti-human growth hormone serum, but complement fixation curves detect the existence of at least two populations of antibodies presumably originated against the sequence 73-128 of human growth hormone. Of these, only one of the corresponding antigenic areas is present in the homologous region of equine growth hormone. The known cross-reactivity detected between both hormones is thus partially explained.
对包含73至128位氨基酸的合成人生长激素片段以及由73至123位氨基酸形成的同源天然马生长片段的免疫特性进行了比较研究。使用了用天然人生长激素或这些片段接种兔子后获得的抗血清。通过血凝实验，两个片段对抗人生长激素血清具有相同的反应性，但补体结合曲线检测到至少存在两种可能针对人生长激素73 - 128序列产生的抗体群体。其中，只有一个相应的抗原区域存在于马生长激素的同源区域。因此，这两种激素之间已知的交叉反应性得到了部分解释。
