Noble R L, Yamashiro D, Li C H
Int J Pept Protein Res. 1977 Nov;10(5):385-93.
The amino terminal 54 residue peptide fragment of human somatotropin [Cys(Gam)53]-HGH-(1-54), has been synthesized by the solid-phase method. The symmetrical anhydride and active ester coupling methods were used exclusively. The synthetic product was purified by gel fitration isoelectric focusing, and partition chromatography. It was found to be homogeneous by six additional criteria. In complement fixation experiments the synthetic product was immunologically active with antisera to HGH and [cys(Cam)53]-HGH-(134). Antiserum raised against the synthetic product was immunologically active in the homologous assay and with HGH,[Cys(Cam)53]-HGH-(1-134), and [Cys(Cam)53]-HGH-(15-125). The synthetic fragment exhibited 53% of the activity of [Cys(Cam)53]-HGH-(1-134) in the rat tibia assay.
人生长激素的氨基末端54个残基的肽片段[Cys(Gam)53]-HGH-(1-54)已通过固相法合成。仅使用了对称酸酐和活性酯偶联方法。合成产物通过凝胶过滤、等电聚焦和分配色谱法进行纯化。通过另外六个标准发现其具有均一性。在补体结合实验中，合成产物与抗HGH和[cys(Cam)53]-HGH-(1-134)的抗血清具有免疫活性。针对合成产物产生的抗血清在同源测定中以及与HGH、[Cys(Cam)53]-HGH-(1-134)和[Cys(Cam)53]-HGH-(15-125)具有免疫活性。在大鼠胫骨试验中，合成片段表现出[Cys(Cam)53]-HGH-(1-134)活性的53%。
