Poskus E, Zakin M M, Fernámdez H N, Paladini A C
Eur J Immunol. 1976 Jun;6(6):409-17. doi: 10.1002/eji.1830060607.
Peptide fragments, obtained from equine growth hormone by cyanogen bromide cleavage and further chemical treatment, were isolated and identified. Their immunological reactivities were tested by hemagglutination and complement fixation methods using rabbit antisera against native hormone. Antigenic determinants were detected in the fragments comprising amino acid sequences 5-72 and 73-123, this last one being predominant. Fragment 124-178 had very low reactivity. Nitration of peptide 73-123 did not modify its immunological properties,but oxidation diminished them. Comparison of the antigenicity of equine growth hormone fragment 73-123 with that of the homologous ovine growth hormone fragment 86-123 lent support to the hypothesis that at least one antigenic determinant area in the horse hormone fragment is dependent on sequence 86-123.
通过溴化氰裂解和进一步化学处理从马生长激素中获得的肽片段被分离和鉴定。使用针对天然激素的兔抗血清，通过血凝和补体结合方法测试它们的免疫反应性。在包含氨基酸序列5 - 72和73 - 123的片段中检测到抗原决定簇，后一个片段占主导。片段124 - 178的反应性非常低。肽73 - 123的硝化没有改变其免疫特性，但氧化使其减弱。将马生长激素片段73 - 123与同源羊生长激素片段86 - 123的抗原性进行比较，支持了以下假设：马激素片段中至少一个抗原决定簇区域依赖于序列86 - 123。
