McMahon V, Stumpf P K
Plant Physiol. 1966 Jan;41(1):148-56. doi: 10.1104/pp.41.1.148.
The capacity of both developing seeds and germinating seedlings of safflower for the incorporation of acetate-C(14) into long-chain fatty acids is examined. Intact tissue of the developing seed shows a low rate of acetate incorporation into fatty acid initially but between the tenth and twenty-fifth day after flowering the tissue has a high rate of synthesis, in particular with respect to the unsaturated fatty acids. Centrifuged fractionation of homogenates of this developmental tissue yielded several active fractions, the most active being the P(I) fraction consisting mostly of plastids. Cofactor requirements and pH effects are examined. Germinating tissue shows a more uniform capacity for synthesis of fatty acids since there is no marked change in synthetic capacity. The newly synthesized fatty acids are consistently palmitic, stearic, and oleic acid. No linoleic synthesis could be detected. The most active fraction of cell-free preparation of germinating tissue is the plastid fraction (P(I)), similar to what was formed with developing tissue.
对红花发育中的种子和萌发的幼苗将乙酸 - C(14) 掺入长链脂肪酸的能力进行了研究。发育中种子的完整组织最初显示出乙酸掺入脂肪酸的速率较低，但在开花后的第十天到第二十五天之间，该组织具有较高的合成速率，特别是对于不饱和脂肪酸而言。对这种发育组织匀浆进行离心分级分离得到了几个活性级分，活性最高的是主要由质体组成的P(I) 级分。研究了辅因子需求和pH效应。萌发组织显示出更均匀的脂肪酸合成能力，因为合成能力没有明显变化。新合成的脂肪酸始终是棕榈酸、硬脂酸和油酸。未检测到亚油酸的合成。萌发组织的无细胞制剂中活性最高的级分是质体级分 (P(I))，这与发育组织形成的情况类似。
