McCabe R M, Donkersloot J A
Infect Immun. 1977 Dec;18(3):726-34. doi: 10.1128/iai.18.3.726-734.1977.
The effect of extracellular products from Streptococcus salivarius on sucrose-dependent adherence to smooth surfaces by other oral bacteria was studied in vitro. Strains of Streptococcus mitis, Streptococcus pyogenes, and Veillonella parvula without innate ability to adhere to a steel wire were able to do so when incubated with sucrose and cell-free culture fluid from S. salivarius strains 9759, 25975, CNII, and MEPI. These culture fluids synthesized more adherent material and water-insoluble glucan than those from Streptococcus mutans C67-1 and seven other S. salivarius strains. Among the S. salivarius strains, glucosyltransferase (GT; dextransucrase, EC 2.4.1.5) activity varied more than 100-fold. Cells of Veillonella and S. mitis S3 that had been incubated in culture fluids from S. salivarius 25975 and 9759, respectively, and then washed adhered upon subsequent incubation with sucrose. This was due to adsorbed GT because (i) the adherence was sensitive to dextranase; (ii) it was observed only with the high-GT culture fluids; (iii) it was dependent on sucrose; and (iv) the washed Veillonella cells synthesized glucan, but not fructan, from sucrose. These results suggest that sucrose-dependent adherence of bacteria without such innate ability can be mediated by (i) entrapment in insoluble glucan synthesized by S. salivarius culture fluids, and (ii) prior adsorption of GT from S. salivarius culture fluids. The possibility that GT formed by high-yield strains of S. salivarius is distributed through the mouth by the action of salivary flow and contributes to sucrose-dependent adherence and plaque formation is considered.
在体外研究了唾液链球菌的细胞外产物对其他口腔细菌蔗糖依赖性黏附于光滑表面的影响。无天然黏附钢丝能力的缓症链球菌、化脓性链球菌和小韦荣球菌菌株，与蔗糖及唾液链球菌9759、25975、CNII和MEPI菌株的无细胞培养液一起孵育时，能够实现这种黏附。这些培养液比变形链球菌C67-1和其他7株唾液链球菌菌株的培养液合成了更多的黏附物质和水不溶性葡聚糖。在唾液链球菌菌株中，葡糖基转移酶（GT；葡聚糖蔗糖酶，EC 2.4.1.5）活性变化超过100倍。分别在唾液链球菌25975和9759的培养液中孵育后洗涤的韦荣球菌和缓症链球菌S3细胞，随后与蔗糖一起孵育时会发生黏附。这是由于吸附了GT，因为（i）黏附对葡聚糖酶敏感；（ii）仅在高GT培养液中观察到；（iii）其依赖于蔗糖；（iv）洗涤后的韦荣球菌细胞从蔗糖合成葡聚糖，但不合成果聚糖。这些结果表明，无此类天然能力的细菌的蔗糖依赖性黏附可通过以下方式介导：（i）被困于唾液链球菌培养液合成的不溶性葡聚糖中，以及（ii）预先从唾液链球菌培养液中吸附GT。考虑到唾液链球菌高产菌株形成的GT可能通过唾液流动作用在口腔中分布，并有助于蔗糖依赖性黏附和菌斑形成。
