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紫外线照射对胶原蛋白折叠形成的影响。

The effect of ultraviolet irradiation on collagen-fold formation.

作者信息

Cooper D R, Davidson R J

出版信息

Biochem J. 1966 Mar;98(3):655-61. doi: 10.1042/bj0980655.

Abstract
  1. A study has been made of the effect of ultraviolet irradiation, in the presence of air and nitrogen, on the conformational changes taking place in cooled solutions of gelatin prepared from thermally denatured neutral-salt-soluble collagen. 2. The increase in negative rotation and viscosity at 15 degrees for irradiated and thermally denatured solutions of collagen becomes less as the irradiation time is increased. 3. The principal effect of ultraviolet irradiation is the fission of the primary collagen peptide chains, eventually yielding chain lengths incapable of stabilizing a helical structure. 4. Irradiation in both air and nitrogen results in a loss of tyrosine, histidine and phenylalanine, with more denaturation occurring in the presence of nitrogen.
摘要
  1. 已经对在空气和氮气存在下紫外线照射对由热变性中性盐溶性胶原蛋白制备的明胶冷却溶液中发生的构象变化的影响进行了研究。2. 随着照射时间的增加,胶原蛋白照射和热变性溶液在15度时负旋光度和粘度的增加变得更小。3. 紫外线照射的主要作用是使原胶原蛋白肽链断裂,最终产生无法稳定螺旋结构的链长。4. 在空气和氮气中照射都会导致酪氨酸、组氨酸和苯丙氨酸的损失,在氮气存在下发生的变性更多。

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本文引用的文献

2
Enzymic studies of the gelatin to collagen-fold transition.
Biochim Biophys Acta. 1959 Dec;36:427-47. doi: 10.1016/0006-3002(59)90185-4.
9
The structure of collagen and gelatin.胶原蛋白和明胶的结构。
Adv Protein Chem. 1961;16:1-138. doi: 10.1016/s0065-3233(08)60028-5.
10
The effect of ions on the kinetics of formation and the stability of the collagenfold.
Biochemistry. 1962 Jul;1:664-74. doi: 10.1021/bi00910a020.

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