大鼠肝脏和胸腺组蛋白的代谢特性。
Metabolic properties of histones from rat liver and thymus gland.
作者信息
Ord M G, Stocken L A
出版信息
Biochem J. 1966 Mar;98(3):888-97. doi: 10.1042/bj0980888.
Abstract
- The incorporation of (14)C-labelled amino acids into acid-extractable proteins from rat-liver and -thymus nuclei confirmed the existence of a protein component with a higher uptake than that into the major histone components. 2. This rapidly labelled component appeared to contain the thiol groups detectable in the acid extracts. 3. Histone f1 contained 1mol. of serine phosphate/mol. of mol.wt. of 42000-43000. 4. Phosphate was present in other components of the 50mm-hydrochloric acid extract from liver and thymus nuclei, and was probably associated with the thiol-containing component. 5. The difference in amino acid uptake into the histones of diffuse and dense chromatin was confirmed. Dense chromatin was found to have a higher proportion of disulphide than did diffuse chromatin.
摘要
- 将（14）C 标记的氨基酸掺入大鼠肝脏和胸腺细胞核的酸可提取物蛋白质中，证实存在一种蛋白质成分，其摄取量高于主要组蛋白成分。2. 这种快速标记的成分似乎含有在酸提取物中可检测到的巯基。3. 组蛋白 f1 每摩尔分子量为 42000 - 43000 含有 1 摩尔丝氨酸磷酸。4. 磷酸盐存在于肝脏和胸腺细胞核 50 毫米盐酸提取物的其他成分中，可能与含巯基成分有关。5. 证实了弥散染色质和致密染色质组蛋白中氨基酸摄取的差异。发现致密染色质的二硫键比例高于弥散染色质。
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