White E R, Carroll M A, Zarembo J E
J Antibiot (Tokyo). 1977 Oct;30(10):811-8. doi: 10.7164/antibiotics.30.811.
Improved methods for the separation and quantitation of cephalosporins, penicillins, tetracyclines and several miscellaneous antibiotics by reverse phase high speed liquid chromatography are presented. The methods have been improved significantly by the substitution of high efficiency, small particle (approximately 10 micrometer reverse phase columns in place of the previously used medium efficiency, pellicular columns. The conditions and procedures described here illustrate that considerable improvements in separation and sensitivity of detection of antibiotics are achieved. Pure compounds, complex mixtures of antibiotics in a variety of dosage forms and fermentation broths are routinely analyzed by the described procedures.
本文介绍了通过反相高效液相色谱法分离和定量头孢菌素、青霉素、四环素及其他几种抗生素的改进方法。通过用高效、小颗粒（约10微米）反相柱替代以前使用的中效、薄壳型柱，这些方法得到了显著改进。这里描述的条件和程序表明，抗生素的分离和检测灵敏度有了相当大的提高。通过所述程序可常规分析纯化合物、各种剂型中的抗生素复杂混合物以及发酵液。
