金黄色葡萄球菌α毒素突变体的生物学特性
Biological properties of alpha-toxin mutants of Staphylococcus aureus.
作者信息
McClatchy J K, Rosenblum E D
出版信息
J Bacteriol. 1966 Sep;92(3):575-9. doi: 10.1128/jb.92.3.575-579.1966.
McClatchy, J. K. (The University of Texas Southwestern Medical School, Dallas), and E. D. Rosenblum. Biological properties of alpha-toxin mutants of Staphylococcus aureus. J. Bacteriol. 92:575-579. 1966.-Twenty nonhemolytic mutants of Staphylococcus aureus were isolated after treatment of a hemolytic strain with ultraviolet light or nitrous acid. Thirteen strains isolated were completely lacking in the synthesis of alpha toxin or immunologically related proteins, presumably the result of a single mutational event. Although the strains were nonhemolytic on rabbit blood-agar plates, six of them retained the dermonecrotic and lethal activities usually associated with staphylococcal alpha toxin, as well as slight hemolytic activity for rabbit erythrocyte suspensions. The active mutants and one inactive mutant produced a protein that reacted immunologically with antibody to alpha toxin. Mutations which alter the alpha toxin molecule can effect the lethal, dermonecrotic, and hemolytic activities separately or in varying ratios.
麦克拉奇，J.K.（得克萨斯大学西南医学中心，达拉斯），以及E.D.罗森布卢姆。金黄色葡萄球菌α毒素突变体的生物学特性。《细菌学杂志》92:575 - 579。1966年。——用紫外线或亚硝酸处理一株溶血菌株后，分离出20株非溶血突变体。分离出的13株菌株完全缺乏α毒素或免疫相关蛋白的合成，推测这是单个突变事件的结果。尽管这些菌株在兔血琼脂平板上不溶血，但其中6株保留了通常与葡萄球菌α毒素相关的皮肤坏死和致死活性，以及对兔红细胞悬液的轻微溶血活性。活性突变体和一个无活性突变体产生了一种与α毒素抗体发生免疫反应的蛋白质。改变α毒素分子的突变可以分别或以不同比例影响致死、皮肤坏死和溶血活性。
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