器官培养中的初次抗体反应。
Primary antibody response in organ cultures.
作者信息
Globerson A, Auerbach R
出版信息
J Exp Med. 1966 Nov 1;124(5):1001-16. doi: 10.1084/jem.124.5.1001.
Abstract
Specific antibody formation has been elicited in vitro following antigenic stimulation by either sheep (in a total of 472 of 875 cultures) or chick erythrocytes (in 65 of 135 cultures tested). The response was manifested by mouse spleen and lymph node explants whereas thymus cultures were inactive. The reaction has been characterized as a primary immune response in view of its kinetics as compared to defined primary and secondary responses, the effect of 2-mercaptoethanol on the antibodies formed, its subject to puromycin inhibition and its sensitivity to X-irradiation. Histological studies revealed preservation of the lymphoid cell populations throughout the entire experimental period.
摘要
在用绵羊（875个培养物中的472个）或鸡红细胞（在测试的135个培养物中的65个）进行抗原刺激后，体外已引发特异性抗体形成。反应表现为小鼠脾脏和淋巴结外植体的反应，而胸腺培养物无活性。鉴于其动力学与明确的初次和二次反应相比、2-巯基乙醇对所形成抗体的影响、其受嘌呤霉素抑制以及对X射线照射的敏感性，该反应被表征为初次免疫反应。组织学研究显示在整个实验期间淋巴细胞群体得以保留。
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