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1
Primary antibody response in organ cultures.器官培养中的初次抗体反应。
J Exp Med. 1966 Nov 1;124(5):1001-16. doi: 10.1084/jem.124.5.1001.
2
Cells involved in the immune response. IV. The response of normal and immune rabbit bone marrow and lymphoid tissue lymphocytes to antigens in vitro.参与免疫反应的细胞。IV. 正常和免疫兔骨髓及淋巴组织淋巴细胞在体外对抗原的反应。
J Exp Med. 1968 Nov 1;128(5):1099-128. doi: 10.1084/jem.128.5.1099.
3
The effects of mercaptoethanol and of peritoneal macrophages on the antibody-forming capacity of nonadherent mouse spleen cells in vitro.巯基乙醇和腹腔巨噬细胞对体外非黏附性小鼠脾细胞抗体形成能力的影响。
J Exp Med. 1972 Sep 1;136(3):604-17. doi: 10.1084/jem.136.3.604.
4
Antibody production by host cells in x-irradiated recipients of allogeneic spleen cells.在接受异体脾细胞X射线照射的受体中宿主细胞产生抗体的情况。
J Immunol. 1970 Oct;105(4):957-63.
5
Cells involved in the immune response. XXIX Establishment of optimal conditions for the primary and secondary immune responses by rabbit lymphoid cells in vitro.参与免疫反应的细胞。二十九 兔淋巴细胞在体外建立初次和二次免疫反应的最佳条件。
Pathol Microbiol (Basel). 1975;42(2):73-91.
6
Radioresistance of the enhancing effect of cells from carrier-immunized mice in an in vitro primary immune response.载体免疫小鼠细胞在体外初次免疫应答中增强作用的放射抗性。
Proc Natl Acad Sci U S A. 1971 Apr;68(4):699-703. doi: 10.1073/pnas.68.4.699.
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In vitro stimulation of antibody formation by peritoneal cells. II. Cell interactions and effects of immunochemical or metabolic inhibitors.腹膜细胞对抗体形成的体外刺激。II. 细胞相互作用以及免疫化学或代谢抑制剂的作用
J Exp Med. 1970 May 1;131(5):917-35. doi: 10.1084/jem.131.5.917.
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Cell to cell interaction in the immune response. V. Target cells for tolerance induction.免疫反应中的细胞间相互作用。五、诱导耐受的靶细胞。
J Exp Med. 1970 Apr 1;131(4):675-99. doi: 10.1084/jem.131.4.675.
9
Restoration of impaired immune functions in aging animals. II. Effect of mercaptoethanol in enhancing the reduced primary antibody responsiveness in vitro.衰老动物受损免疫功能的恢复。II. 巯基乙醇在体外增强降低的初次抗体反应性中的作用。
Mech Ageing Dev. 1979 Jun;10(5):325-40. doi: 10.1016/0047-6374(79)90045-9.
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The effect of antigen competition on both the primary and secondary immune capacity in mice.抗原竞争对小鼠初次和二次免疫能力的影响。
J Immunol. 1970 Jan;104(1):166-77.

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2
Reactivation in vitro of immunocompetence in irradiated mouse spleen.照射鼠脾免疫活性的体外再激活。
J Exp Med. 1967 Aug 1;126(2):223-34. doi: 10.1084/jem.126.2.223.
3
Immunization of dissociated spleen cell cultures from normal mice.对来自正常小鼠的脾细胞解离培养物进行免疫接种。
J Exp Med. 1967 Sep 1;126(3):423-42. doi: 10.1084/jem.126.3.423.
4
Immune responses in vitro. I. Cellular requirements for the immune response by nonprimed and primed spleen cells in vitro.体外免疫反应。I. 体外非致敏和致敏脾细胞免疫反应的细胞需求。
J Exp Med. 1969 Aug 1;130(2):345-64. doi: 10.1084/jem.130.2.345.
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Depressed cellular formation of antibody to Shigella antigens in vitro by spleen cell cultures from unresponsive mice.来自无反应小鼠的脾细胞培养物在体外对志贺氏菌抗原的抗体细胞形成受到抑制。
J Bacteriol. 1968 Oct;96(4):1124-32. doi: 10.1128/jb.96.4.1124-1132.1968.
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In vitro induction of a primary response to the dinitrophenyl determinant.对二硝基苯基决定簇的初次反应的体外诱导
J Exp Med. 1970 Jan 1;131(1):93-9. doi: 10.1084/jem.131.1.93.
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In vitro and in vivo studies of the immune response to sheep erythrocytes using partially purified cell preparations.使用部分纯化的细胞制剂对绵羊红细胞免疫反应的体外和体内研究。
J Exp Med. 1970 Jan 1;131(1):57-76. doi: 10.1084/jem.131.1.57.
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Chemical markers of transplantation individuality solubilized with sonic energy.用声能溶解的移植个体化学标记物。
Bacteriol Rev. 1971 Mar;35(1):59-85. doi: 10.1128/br.35.1.59-85.1971.
9
[Antibody producers].[抗体产生者]
Klin Wochenschr. 1969 Oct 1;47(19):1019-25. doi: 10.1007/BF01486617.
10
Effect of splenectomy on the immune response in the lizard, Calotes versicolor.脾切除对变色树蜥免疫反应的影响。
Experientia. 1972 Oct 15;28(10):1225-6. doi: 10.1007/BF01946185.

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THE PRODUCTION OF ANTIBODIES BY TISSUES LIVING OUTSIDE OF THE ORGANISM.组织外活体组织产生抗体。
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IMMUNOGENICITY OF ANTIGEN-CONTAINING RIBONUCLEIC ACID PREPARATIONS FROM MACROPHAGES.来自巨噬细胞的含抗原核糖核酸制剂的免疫原性
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ANTIBODY PLAQUE FORMATION BY NORMAL MOUSE SPLEEN CELL CULTURES EXPOSED IN VITRO TO RNA FROM IMMUNE MICE.体外暴露于免疫小鼠RNA的正常小鼠脾细胞培养物形成抗体斑块
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ANTIBODY FORMATION BY RABBIT LYMPH NODE CELLS. IV. THE DETAILED METHODS FOR MEASURING ANTIBODY SYNTHESIS BY INDIVIDUAL CELLS, THE KINETICS OF ANTIBODY FORMATION BY RABBITS AND THE PROPERTIES OF CELL SUSPENSIONS.兔淋巴结细胞的抗体形成。IV. 单个细胞抗体合成的详细测量方法、兔抗体形成的动力学以及细胞悬液的特性
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CYTOPLASMIC INTERACTION BETWEEN MACROPHAGES AND LYMPHOCYTIC CELLS IN ANTIBODY SYNTHESIS.抗体合成中巨噬细胞与淋巴细胞之间的细胞质相互作用
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The role of protein synthesis in the eclipse period of newcastle disease virus multiplication in HeLa cells as studied with puromycin.用嘌呤霉素研究蛋白质合成在新城疫病毒在HeLa细胞中增殖的隐蔽期的作用。
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In vitro transfer of macrophage RNA to lymph node cells.巨噬细胞RNA向淋巴结细胞的体外转移。
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器官培养中的初次抗体反应。

Primary antibody response in organ cultures.

作者信息

Globerson A, Auerbach R

出版信息

J Exp Med. 1966 Nov 1;124(5):1001-16. doi: 10.1084/jem.124.5.1001.

DOI:10.1084/jem.124.5.1001
PMID:5926296
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2138273/
Abstract

Specific antibody formation has been elicited in vitro following antigenic stimulation by either sheep (in a total of 472 of 875 cultures) or chick erythrocytes (in 65 of 135 cultures tested). The response was manifested by mouse spleen and lymph node explants whereas thymus cultures were inactive. The reaction has been characterized as a primary immune response in view of its kinetics as compared to defined primary and secondary responses, the effect of 2-mercaptoethanol on the antibodies formed, its subject to puromycin inhibition and its sensitivity to X-irradiation. Histological studies revealed preservation of the lymphoid cell populations throughout the entire experimental period.

摘要

在用绵羊（875个培养物中的472个）或鸡红细胞（在测试的135个培养物中的65个）进行抗原刺激后，体外已引发特异性抗体形成。反应表现为小鼠脾脏和淋巴结外植体的反应，而胸腺培养物无活性。鉴于其动力学与明确的初次和二次反应相比、2-巯基乙醇对所形成抗体的影响、其受嘌呤霉素抑制以及对X射线照射的敏感性，该反应被表征为初次免疫反应。组织学研究显示在整个实验期间淋巴细胞群体得以保留。