关于在体外短期实验中产生抗绵羊红细胞溶血抗体的细胞的研究。
Studies on cells producing haemolytic antibodies against sheep red blood cells in short-term experiments in vitro.
作者信息
Strander H
出版信息
Immunology. 1966 Jan;10(1):45-55.
Abstract
A system is described for the maintenance of isolated cells from mouse spleens during short periods of time . Active γM antibody synthesis continues throughout the experimental period. The number of antibody-producing cells remains unchanged when the synthesis of DNA is inhibited. The effects of puromycin, parafluorophenylalanine, actinomycin D and hydrocortisone have also been tested and their dose-dependent effects established. γM antibody synthesis is shown to follow the same pattern as mammalian protein synthesis in general.
摘要
描述了一种用于在短时间内维持从小鼠脾脏分离出的细胞的系统。在整个实验期间，活性γM抗体合成持续进行。当DNA合成受到抑制时，产生抗体的细胞数量保持不变。还测试了嘌呤霉素、对氟苯丙氨酸、放线菌素D和氢化可的松的作用，并确定了它们的剂量依赖性效应。总体而言，γM抗体合成显示出与哺乳动物蛋白质合成相同的模式。
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引用本文的文献
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Antibody production in vitro. II. Effects of actinomycin D and puromycin on the secondary response to sheep erythrocytes.体外抗体产生。II. 放线菌素D和嘌呤霉素对绵羊红细胞二次反应的影响。
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INHIBITION BY PUROMYCIN OF THE INITIATION OF SYNTHESIS OF INFECTIOUS RNA AND VIRUS BY CHICKEN EMBRYO CELLS INFECTED WITH WESTERN EQUINE ENCEPHALOMYELITIS VIRUS.嘌呤霉素对感染西部马脑脊髓炎病毒的鸡胚细胞合成传染性RNA及病毒起始过程的抑制作用。
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ACTINOMYCIN: CORRELATION OF STRUCTURE AND FUNCTION OF ITS COMPLEXES WITH PURINES AND DNA.放线菌素：其与嘌呤及DNA复合物的结构与功能的相关性
Science. 1964 Feb 14;143(3607):684-9. doi: 10.1126/science.143.3607.684.
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Proc Natl Acad Sci U S A. 1963 Sep;50(3):436-46. doi: 10.1073/pnas.50.3.436.
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Conditions for the replication of infectious viral RNA.传染性病毒RNA复制的条件。
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