Loewi G, Holborow E J, Temple A
Immunology. 1966 Apr;10(4):339-47.
Guinea-pigs were immunized by injections of blood group substance with incomplete adjuvant, followed after an interval of approximately 2 weeks, by intracutaneous immunization with the same antigen and Freund's adjuvant containing . This treatment inhibited the appearance of delayed skin reactions, while circulating antibody production took place as in controls which had received complete adjuvant only with blood group substance, and had delayed skin reactions. The inhibition of the skin reaction was found to be antigen-specific with regard to unrelated antigens, but showed cross-inhibition for serologically different human blood group substances. The first immunization had to be given more than 2 days before the immunization with complete adjuvant. A similar phenomenon was seen with ovalbumin as antigen. In addition to inhibition of the delayed skin reaction, there appeared to be less γ-antibody to ovalbumin than in ovalbumin plus complete adjuvant-only controls. Passive administration of antibody did not affect the development of a delayed hypersensitivity state in complete adjuvant-immunized animals with blood group substance or ovalbumin as antigen. Present evidence favours an explanation of the phenomenon in terms of temporary paralysis on the part of some of the antibody-producing cells—viz. those concerned with delayed hypersensitivity and γ-antibody production.
用不完全佐剂注射血型物质对豚鼠进行免疫，间隔约2周后，再用相同抗原和含弗氏佐剂进行皮内免疫。这种处理抑制了迟发性皮肤反应的出现，而循环抗体的产生与仅接受血型物质和完全佐剂的对照动物相同，且有迟发性皮肤反应。发现皮肤反应的抑制对于不相关抗原具有抗原特异性，但对血清学不同的人类血型物质表现出交叉抑制。第一次免疫必须在使用完全佐剂免疫前2天以上进行。以卵清蛋白为抗原时也观察到类似现象。除了抑制迟发性皮肤反应外，与仅使用卵清蛋白加完全佐剂的对照相比，针对卵清蛋白的γ抗体似乎更少。被动给予抗体并不影响以血型物质或卵清蛋白为抗原、用完全佐剂免疫的动物迟发型超敏状态的发展。目前的证据支持用部分抗体产生细胞暂时麻痹来解释这一现象，即那些与迟发性超敏反应和γ抗体产生有关的细胞。
