Oh J O, Gill E J
J Bacteriol. 1966 Jan;91(1):251-6. doi: 10.1128/jb.91.1.251-256.1966.
Oh, J. O. (University of British Columbia, Vancouver, B.C., Canada), and E. J. Gill. Role of interferon-like viral inhibitor in endotoxin-induced corneal resistance to Newcastle disease virus. J. Bacteriol. 91:251-256. 1966.-A state of marked resistance to the toxic corneal effects of Newcastle disease virus (NDV) was observed in rabbit eyes after intravenous injection of 10 or 100 mug of typhoid endotoxin. By use of tissue cultures of rabbit corneal endothelial cells for assay, high titers of an interferon-like viral inhibitor were detected in serum and in ocular aqueous humor of these animals. The pretreatment of eyes with aqueous humor or serum containing the inhibitor markedly suppressed the production of corneal toxicity by NDV. The intravenous injection of 1 mug of the endotoxin had a negligible effect on the corneal reaction, and little or no inhibitor was found in either serum or aqueous humor. Normal aqueous humor or serum contained no inhibitor and had no suppressive effect on the NDV-induced reactions. The results indicated that the inhibitor played an important role in the induction of corneal resistance to NDV in vivo. The inhibitor in aqueous humor of endotoxin-injected rabbits was found to be derived from blood after an increase in the permeability of "blood-aqueous barrier" of iris due to the endotoxin. Therefore, intravenously administered typhoid endotoxin induced corneal resistance to NDV in rabbits through its dual action on host: (i) release of an interferon-like viral inhibitor into the blood stream and (ii) disruption of the blood-aqueous barrier of the iris, thus allowing the passage of the viral inhibitor from blood into the anterior chamber, where it modified the corneal endothelial cells to render them resistant to NDV.
哦，J.O.（加拿大不列颠哥伦比亚省温哥华市英属哥伦比亚大学）和E.J.吉尔。类干扰素病毒抑制剂在内毒素诱导的角膜对新城疫病毒抗性中的作用。《细菌学杂志》91:251 - 256。1966年。——静脉注射10或100微克伤寒内毒素后，在兔眼中观察到对新城疫病毒（NDV）毒性角膜效应的显著抗性状态。通过使用兔角膜内皮细胞的组织培养物进行检测，在这些动物的血清和眼房水中检测到高滴度的类干扰素病毒抑制剂。用含有该抑制剂的房水或血清预处理眼睛，可显著抑制NDV产生的角膜毒性。静脉注射1微克内毒素对角膜反应的影响可忽略不计，在血清或房水中几乎未发现抑制剂。正常房水或血清不含抑制剂，对NDV诱导的反应无抑制作用。结果表明，该抑制剂在体内诱导角膜对NDV的抗性中起重要作用。发现注射内毒素的兔房水中的抑制剂是由于内毒素导致虹膜“血 - 房水屏障”通透性增加后从血液中衍生而来。因此，静脉注射的伤寒内毒素通过对宿主的双重作用诱导兔角膜对NDV的抗性：（i）向血流中释放类干扰素病毒抑制剂；（ii）破坏虹膜的血 - 房水屏障，从而使病毒抑制剂从血液进入前房，在那里它修饰角膜内皮细胞使其对NDV具有抗性。
