木霉的β-葡萄糖苷酶：其生物合成及在纤维素糖化中的作用
Beta-glucosidase of Trichoderma: its biosynthesis and role in saccharification of cellulose.
作者信息
Sternberg D
出版信息
Appl Environ Microbiol. 1976 May;31(5):648-54. doi: 10.1128/aem.31.5.648-654.1976.
Abstract
The extracellular beta-glucosidase of Trichoderma viride generally is present in low levels when the organism is cultured on cellulose because it is inactivated under the acid conditions which develop in the medium while the other enzymes of the cellulase complex are more stable. With the appropriate pH control, inactivation of beta-glucosidase is prevented and the activity of this enzyme increases during growth. In the saccharification of crystalline cellulose, or of cellulose at low concentrations, much of the glucose produced is the result of the cleavage of cellobiose by beta-glucosidase. However when high concentrations (10%) of pretreated cellulose are saccharified, significant quantities of glucose are produced by action of enzymes other than beta-glucosidase.
摘要
当绿色木霉在纤维素上培养时，其胞外β-葡萄糖苷酶的含量通常较低，因为在培养基中产生的酸性条件下它会失活，而纤维素酶复合体的其他酶则更稳定。通过适当的pH控制，可以防止β-葡萄糖苷酶失活，并且该酶的活性在生长过程中会增加。在结晶纤维素或低浓度纤维素的糖化过程中，产生的大部分葡萄糖是由β-葡萄糖苷酶裂解纤维二糖的结果。然而，当对高浓度（10%）的预处理纤维素进行糖化时，除β-葡萄糖苷酶之外的其他酶的作用会产生大量葡萄糖。
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