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通过凝胶内局部溶血法（LHG 测定法）研究抗体产生。II. 测定程序。

Antibody production studied by means of the localized haemolysis in gel (LHG) assay. II. Assay procedure.

作者信息

Wortis H H, Taylor R B, Dresser D W

出版信息

Immunology. 1968 Jan;14(1):69-79.

PMID:5635754
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1409262/
Abstract

The localized haemolysis in gel (LHG or plaque) assay has been investigated and it has been shown that the best condition for the assay is a plate made up from a standard tissue culture medium (199) in an agarose gel. Several convenient but sub-optimal alternatives are described. Other variables that were investigated were the concentration of indicator erythrocytes in the assay plate, the amount of complement added, the incubation time and the neutralization of xenogeneic developing serum by free mouse serum γ-globulins. The reasons for using log(+1) transformed data for statistical analysis are also presented.

摘要

已对凝胶中的局部溶血（LHG或噬斑）试验进行了研究，结果表明该试验的最佳条件是使用由标准组织培养基（199）制成的琼脂糖凝胶平板。文中描述了几种方便但次优的替代方法。还研究了其他变量，包括试验平板中指示红细胞的浓度、补体添加量、孵育时间以及游离小鼠血清γ球蛋白对异种发育血清的中和作用。文中还阐述了使用对数（+1）转换数据进行统计分析的原因。

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Immunology. 1968 Jan;14(1):69-79.
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A PLAQUE TECHNIQUE FOR COUNTING CELLS WHICH PRODUCE ANTIBACTERIAL ANTIBODY.一种用于计数产生抗菌抗体细胞的噬菌斑技术。
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ELECTROPHORETIC SEPARATION AND PROPERTIES OF MOUSE ANTIHAPTEN ANTIBODIES INVOLVED IN PASSIVE CUTANEOUS ANAPHYLAXIS AND PASSIVE HEMOLYSIS.参与被动皮肤过敏反应和被动溶血的小鼠抗半抗原抗体的电泳分离及特性
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Antibody production studied by means of the LHG assay. I. The splenic response of CBA mice to sheep erythrocytes.通过淋巴细胞转化试验研究抗体产生。I. CBA小鼠对绵羊红细胞的脾脏反应。
Immunology. 1966 Dec;11(6):603-16.
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Humoral and cellular aspects of the immune response to the somatic antigen of Salmonella enteritidis.针对肠炎沙门氏菌菌体抗原的免疫反应的体液和细胞方面
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19S antibody production against soluble lipopolysaccharide antigens by individual lymphoid cells in vitro.体外单个淋巴细胞针对可溶性脂多糖抗原产生19S抗体。
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