Suppr超能文献

通过凝胶内局部溶血法（LHG 测定法）研究抗体产生。II. 测定程序。

Antibody production studied by means of the localized haemolysis in gel (LHG) assay. II. Assay procedure.

作者信息

Wortis H H, Taylor R B, Dresser D W

出版信息

Immunology. 1968 Jan;14(1):69-79.

PMID:5635754
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1409262/
Abstract

The localized haemolysis in gel (LHG or plaque) assay has been investigated and it has been shown that the best condition for the assay is a plate made up from a standard tissue culture medium (199) in an agarose gel. Several convenient but sub-optimal alternatives are described. Other variables that were investigated were the concentration of indicator erythrocytes in the assay plate, the amount of complement added, the incubation time and the neutralization of xenogeneic developing serum by free mouse serum γ-globulins. The reasons for using log(+1) transformed data for statistical analysis are also presented.

摘要

已对凝胶中的局部溶血（LHG或噬斑）试验进行了研究，结果表明该试验的最佳条件是使用由标准组织培养基（199）制成的琼脂糖凝胶平板。文中描述了几种方便但次优的替代方法。还研究了其他变量，包括试验平板中指示红细胞的浓度、补体添加量、孵育时间以及游离小鼠血清γ球蛋白对异种发育血清的中和作用。文中还阐述了使用对数（+1）转换数据进行统计分析的原因。

相似文献

1
Antibody production studied by means of the localized haemolysis in gel (LHG) assay. II. Assay procedure.通过凝胶内局部溶血法（LHG 测定法）研究抗体产生。II. 测定程序。
Immunology. 1968 Jan;14(1):69-79.
2
Antibody production studied by means of the LHG assay. I. The splenic response of CBA mice to sheep erythrocytes.通过淋巴细胞转化试验研究抗体产生。I. CBA小鼠对绵羊红细胞的脾脏反应。
Immunology. 1966 Dec;11(6):603-16.
3
Antibody production studied by means of the localized haemolysis in gel (LHG) assay. 3. Mouse cells producing five different classes of antibody.通过凝胶局部溶血（LHG）试验研究抗体产生。3. 产生五种不同类别抗体的小鼠细胞。
Immunology. 1969 Jul;17(1):93-110.
4
Antibody formation against 2,4-dinitrophenyl-hapten at the cellular level. Hemolytic plaque-in-gel assay for lymphoid cells producing anti-2,4-dinitrophenyl-hapten antibody.细胞水平上针对2,4-二硝基苯基半抗原的抗体形成。用于产生抗2,4-二硝基苯基半抗原抗体的淋巴细胞的凝胶溶血空斑测定。
J Immunol. 1969 Aug;103(2):357-63.
5
[Agar quality and diethylaminoethyl-dextran as critical component in the LHG-technic].[琼脂质量和二乙氨基乙基葡聚糖作为LHG技术中的关键成分]
Z Immunitatsforsch Allerg Klin Immunol. 1968 Aug;136(2):145-57.
6
Antibody response in rainbow trout (Salmo gairdneri) II. Studies on the kinetics of development of antibody-producing cells and on complement and natural hemolysin.虹鳟（萨氏虹鳟）的抗体反应II. 抗体产生细胞发育动力学以及补体和天然溶血素的研究
J Immunol. 1969 May;102(5):1202-7.
7
[Effect of rubomycin B and C on formation of hemagglutinins and on the content of antibody-forming cells in the spleen of mice, following administration of sheep erythrocytes as antigen].[以绵羊红细胞为抗原给药后，柔红霉素B和C对小鼠脾脏中血凝素形成及抗体形成细胞含量的影响]
Antibiotiki. 1968 Nov;13(11):1022-6.
8
A new system of the indirect localized haemolysis in gel assay.凝胶试验中间接局部溶血的新系统。
Folia Biol (Praha). 1969;15(2):154-6.
9
Recruitment and proliferation of immunocompetent cells during the log phase of the primary antibody response.初次抗体反应对数期免疫活性细胞的募集与增殖。
J Immunol. 1969 Oct;103(4):668-78.
10
Dynamics of the immune reaction in chickens studied by means of localized haemolysis in gel.
Folia Biol (Praha). 1967;13(4):317-9.

引用本文的文献

1
Effect of the immunosuppressive agent ovalicin on the kinetics of antibody formation.免疫抑制剂椭圆玫瑰树碱对抗体形成动力学的影响。
Agents Actions. 1970 Dec;1(5):221-6. doi: 10.1007/BF01968693.
2
Immune responses in vitro. 3. Development of primary gamma-M, gamma-G, and gamma-A plaque-forming cell responses in mouse spleen cell cultures stimulated with heterologous erythrocytes.体外免疫反应。3. 用异种红细胞刺激小鼠脾细胞培养物中主要γ-M、γ-G和γ-A斑块形成细胞反应的发展。
J Exp Med. 1971 Aug 1;134(2):395-416. doi: 10.1084/jem.134.2.395.
3
The effect of pertussis vaccine on the immune response of mice to sheep red blood cells.百日咳疫苗对小鼠针对绵羊红细胞免疫反应的影响。
Clin Exp Immunol. 1970 Dec;7(6):817-31.
4
Allotypic suppression of adult mouse spleen cells.成年小鼠脾细胞的同种异型抑制
Immunology. 1970 Jul;19(1):169-79.
5
Modulation of the immune response by a methanol-insoluble fraction of attenuated tubercle bacilli (BCG). I. Primary and secondary responses to sheep red blood cells and T2 phage.减毒结核杆菌（卡介苗）甲醇不溶部分对免疫反应的调节作用。I. 对绵羊红细胞和T2噬菌体的初次和二次反应
Clin Exp Immunol. 1970 Aug;7(2):269-81.
6
The induction of an immune response in vitro to a protein antigen (rabbit Fab'2).在体外对蛋白质抗原（兔Fab'2）诱导免疫反应。
Immunology. 1970 Sep;19(3):491-9.
7
Regulation of antibody synthesis against Escherichia coli endotoxin. IV. Induction of paralysis in vitro by treating normal lymphoid cells with antigen.抗大肠杆菌内毒素抗体合成的调节。IV. 用抗原处理正常淋巴细胞在体外诱导麻痹。
J Exp Med. 1969 Mar 1;129(3):469-82. doi: 10.1084/jem.129.3.469.
8
The relationship between the immunoglobulin class of B-cell precursors and the degree of synergism obtained from the presence of T cells.B细胞前体的免疫球蛋白类别与T细胞存在所产生的协同作用程度之间的关系。
Clin Exp Immunol. 1974 Mar;16(3):393-400.
9
Response of mice to rabbit Fab' 2 and Fab'. Thymus independence of IgM response and thymus dependence of IgG response.小鼠对兔F(ab')₂和Fab'的反应。IgM反应的胸腺非依赖性及IgG反应的胸腺依赖性。
Immunology. 1972 Jul;23(1):69-80.
10
Specific antibody-mediated effect on the immune response. Suppression and augmentation of the primary immune response in mice by different classes of antibodies.特异性抗体介导的对免疫反应的影响。不同类别抗体对小鼠初次免疫反应的抑制和增强作用。
Immunology. 1972 Mar;22(3):319-31.

本文引用的文献

1
A PLAQUE TECHNIQUE FOR COUNTING CELLS WHICH PRODUCE ANTIBACTERIAL ANTIBODY.一种用于计数产生抗菌抗体细胞的噬菌斑技术。
Aust J Exp Biol Med Sci. 1965 Apr;43:163-6. doi: 10.1038/icb.1965.13.
2
ELECTROPHORETIC SEPARATION AND PROPERTIES OF MOUSE ANTIHAPTEN ANTIBODIES INVOLVED IN PASSIVE CUTANEOUS ANAPHYLAXIS AND PASSIVE HEMOLYSIS.参与被动皮肤过敏反应和被动溶血的小鼠抗半抗原抗体的电泳分离及特性
J Exp Med. 1964 Aug 1;120(2):315-28. doi: 10.1084/jem.120.2.315.
3
APPLICATION OF A LOCALIZED HEMOLYSIN REACTION FOR SPECIFIC DETECTION OF INDIVIDUAL ANTIBODY-FORMING CELLS.局部溶血素反应在个体抗体形成细胞特异性检测中的应用。
J Exp Med. 1964 Apr 1;119(4):667-84. doi: 10.1084/jem.119.4.667.
4
LOW TEMPERATURE PRESERVATION OF MOUSE LYMPHOCYTES WITH DIMETHYL SULFOXIDE.用二甲基亚砜对小鼠淋巴细胞进行低温保存。
Blood. 1964 Apr;23:494-501.
5
Studies in the variability of pock counts.痘疱计数变异性的研究。
J Hyg (Lond). 1957 Dec;55(4):564-81. doi: 10.1017/s0022172400037451.
6
Antibody production studied by means of the LHG assay. I. The splenic response of CBA mice to sheep erythrocytes.通过淋巴细胞转化试验研究抗体产生。I. CBA小鼠对绵羊红细胞的脾脏反应。
Immunology. 1966 Dec;11(6):603-16.
7
A mathematical model relating circulating antibody and antibody forming cells.
J Immunol. 1966 Jul;97(1):34-40.
8
A method of increased sensitivity for detecting single antibody-forming cells.一种提高检测单个抗体形成细胞灵敏度的方法。
Nature. 1965 Sep 4;207(5001):1106-7. doi: 10.1038/2071106a0.
9
Humoral and cellular aspects of the immune response to the somatic antigen of Salmonella enteritidis.针对肠炎沙门氏菌菌体抗原的免疫反应的体液和细胞方面
J Exp Med. 1965 Sep 1;122(3):483-504. doi: 10.1084/jem.122.3.483.
10
19S antibody production against soluble lipopolysaccharide antigens by individual lymphoid cells in vitro.体外单个淋巴细胞针对可溶性脂多糖抗原产生19S抗体。
Nature. 1965 Sep 11;207(5002):1166-8. doi: 10.1038/2071166a0.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验