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大鼠睾丸血流测量的方法学方面

Methodological aspects of testicular blood flow measurements in rats.

作者信息

Damber J E, Janson P O

出版信息

Acta Physiol Scand. 1977 Nov;101(3):278-85. doi: 10.1111/j.1748-1716.1977.tb06009.x.

DOI:10.1111/j.1748-1716.1977.tb06009.x
PMID:596203
Abstract

3 techniques for the measurement of testicular blood flow in anesthetized adult rats were compared. Direct measurement of testicular venous outflow yielded values more than 3 times lower than those obtained Xe-133 clearance and radioactive microsphere techniques due to the surgical procedures involved in spermatic venous cannulation. There was an agreement between flow values obtained with Xe-133 clearance (17.8 +/- 3.5 ml/100 g X min) and radioactive microspheres (19.9 +/- 5.5 ml/100 g X min). A homogeneous distribution of microspheres to different segments of the testis indicates that Xe-133 clearance is an adequate technique for testicular blood flow measurements. However, for some experimental purposes the radioactive microsphere technique is more versatile than Xe-133 clearance because of its capacity of measuring several organ flows simultaneously.

摘要

比较了3种测量麻醉成年大鼠睾丸血流量的技术。由于精索静脉插管所涉及的外科手术,直接测量睾丸静脉流出量得到的值比用Xe - 133清除法和放射性微球技术获得的值低3倍以上。用Xe - 133清除法(17.8±3.5 ml/100 g×分钟)和放射性微球法(19.9±5.5 ml/100 g×分钟)获得的血流量值之间具有一致性。微球在睾丸不同节段的均匀分布表明,Xe - 133清除法是测量睾丸血流量的一种合适技术。然而,对于某些实验目的,放射性微球技术比Xe - 133清除法更通用,因为它能够同时测量多个器官的血流量。

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Methodological aspects of testicular blood flow measurements in rats.大鼠睾丸血流测量的方法学方面
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3
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7
Ipsilateral and contralateral testicular blood flows during unilateral testicular torsion by 133Xe clearance technique.
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