人尿中17-氧代类固醇葡糖苷酸酯和硫酸盐的间接分析。
The indirect analysis of 17-oxosteroid glucosiduronates and sulphates in human urine.
作者信息
Menini E
出版信息
Biochem J. 1966 Jun;99(3):747-59. doi: 10.1042/bj0990747.
Abstract
- An analytical procedure has been developed for the determination of urinary 17-oxosteroid sulphates and 11-deoxy-17-oxosteroid glucosiduronates. 2. By submitting urine to the following operations: (a) extraction of the 17-oxosteroid conjugates into chloroform (in the presence of pyridinium sulphate), (b) solvolysis (in the absence of acid) of the steroid sulphates, (c) separation of the steroid glucosiduronates, (d) hydrolysis of the glucosiduronates, (e) oxidation with tert.-butyl chromate of the liberated steroids and (f) analysis of the final extracts by gas-liquid chromatography, the 17-oxosteroid conjugates are converted into and measured as androstanediones and androstane-(ene)triones. 3. Values for the daily excretion of 17-oxosteroid glucosiduronates and sulphates in normal men and women are given.
摘要
- 已开发出一种分析方法，用于测定尿中17-氧代类固醇硫酸盐和11-脱氧-17-氧代类固醇葡糖苷酸。2. 通过对尿液进行以下操作：(a) 将17-氧代类固醇结合物萃取到氯仿中（在硫酸吡啶存在下），(b) 类固醇硫酸盐的溶剂解（在无酸条件下），(c) 类固醇葡糖苷酸的分离，(d) 葡糖苷酸的水解，(e) 用叔丁基铬酸酯氧化释放出的类固醇，以及(f) 通过气液色谱法分析最终提取物，17-氧代类固醇结合物被转化为雄甾烷二酮和雄甾烷（烯）三酮并进行测定。3. 给出了正常男性和女性尿中17-氧代类固醇葡糖苷酸和硫酸盐的每日排泄量值。
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