Veerkamp J H
Antonie Van Leeuwenhoek. 1977;43(2):101-10. doi: 10.1007/BF00395665.
Lipid-phosphorus and lipid-galactose content and phospholipid and fatty acid composition of Bifidobacterium bifidum subsp. pennsylvanicum were examined under a wide variety of growth conditions. Cells from 29-C cultures contained less lipid-phosphorus than did cells from 37-C cultures, but their lipid-galactose content and phospholipid composition did not differ. At both temperatures, the growth phase influenced the lipid composition similarly. Phosphate, Mg2+ and K+ concentrations in the medium did neither significantly change the cellular lipid-phosphorus content nor the phospholipid composition. Only Mg2+-deficiency markedly reduced growth and lowered the content of cellular lipid-galactose. Omission of Tween 80 from the medium did not affect growth, but lowered the content of lipid-galactose and augmented those of lipid-phosphorus and diphosphatidylglycerol in the cell. Increased osmolarity and substitution of other Tween for Tween 80 caused the same changes in lipid composition, and besides inhibited growth. Omitting Tween 80 and replacing it by other Tweens dramatically reduced the percentage of unsaturated fatty acids. C12- and C14-fatty acids made up about 50% of total fatty acids in cells from Tween 20 cultures and 12-14% in cells from Tween 40 and Tween 60 cultures. The differences in the decline of unsaturated fatty acids and in the degree of replacement of these acids by C12- and C14-fatty acids may be related to the variations in growth in cultures with various Tweens by way of changes in the physical state of the membrane lipids.
在多种生长条件下，对宾夕法尼亚双歧杆菌亚种的脂质磷、脂质半乳糖含量以及磷脂和脂肪酸组成进行了检测。来自29℃培养物的细胞所含脂质磷比37℃培养物的细胞少，但它们的脂质半乳糖含量和磷脂组成并无差异。在这两个温度下，生长阶段对脂质组成的影响相似。培养基中的磷酸盐、Mg2+和K+浓度既没有显著改变细胞脂质磷含量，也没有改变磷脂组成。只有Mg2+缺乏显著降低了生长并降低了细胞脂质半乳糖的含量。从培养基中省略吐温80不影响生长，但降低了脂质半乳糖的含量，并增加了细胞中脂质磷和二磷脂酰甘油的含量。渗透压增加以及用其他吐温替代吐温80导致脂质组成发生相同变化，并且还抑制了生长。省略吐温80并用其他吐温替代它会显著降低不饱和脂肪酸的百分比。在吐温20培养物的细胞中，C12和C14脂肪酸约占总脂肪酸的50%，在吐温40和吐温60培养物的细胞中占12 - 14%。不饱和脂肪酸下降以及这些酸被C12和C14脂肪酸替代程度的差异可能与不同吐温培养物中生长的变化有关，这是通过膜脂物理状态的改变实现的。
