Veerkamp J H
Antonie Van Leeuwenhoek. 1977;43(2):111-24. doi: 10.1007/BF00395666.
The cation content of Bifidobacterium bifidum subsp. pennsylvanicum was markedly influenced by the washing procedure of the cells, by the growth phase and the temperature, and by the composition of the culture medium. Optimal retention of cations was achieved by washing with 0.25 M MgCl2 at 20C. The intracellular Na+ concentration rose during growth in normal medium to a constant value in the stationary phase, the K+ concentration rose in the exponential phase, but fell in the stationary phase. Cells from 29-C cultures contained more Na+ and less K+ in the stationary phase than did cells from 37-C cultures, but the total cation content was the same at 29 and 37C. Intracellular Na+ and K+ concentrations were dependent on the concentrations in the medium and on its osmolarity. The intracellular Na+/K+ ratio varied from 0.04 to 2.3. The concentrations of Na+,K+ and phosphate in the medium hardly affected growth. Mg2+-deficiency of the medium markedly decreased the concentration of Mg2+ within the cell; its concentration in the cell sap was greatly affected, but the amount of sedimentable, bound Mg2+ only slightly. The content of K+ within the cell decreased in Mg2+-deficient medium, but the concentration of Na+ did not. Omission of Tween 80 as well as its substitution by Tween 20 caused a decrease of intracellular K+. Cells from Tween 40 and Tween 60 cultures additionally contained markedly less Na+.
宾夕法尼亚双歧杆菌亚种的阳离子含量受到细胞洗涤程序、生长阶段、温度以及培养基组成的显著影响。通过在20℃下用0.25M MgCl₂洗涤可实现阳离子的最佳保留。在正常培养基中生长期间，细胞内Na⁺浓度在稳定期升至恒定值，K⁺浓度在指数期上升，但在稳定期下降。与37℃培养的细胞相比，29℃培养的细胞在稳定期含有更多的Na⁺和更少的K⁺，但29℃和37℃时的总阳离子含量相同。细胞内Na⁺和K⁺浓度取决于培养基中的浓度及其渗透压。细胞内Na⁺/K⁺比值在0.04至2.3之间变化。培养基中Na⁺、K⁺和磷酸盐的浓度对生长影响不大。培养基中Mg²⁺缺乏显著降低了细胞内Mg²⁺的浓度；其在细胞液中的浓度受到很大影响，但可沉淀的结合Mg²⁺量仅略有影响。在Mg²⁺缺乏的培养基中，细胞内K⁺含量降低，但Na⁺浓度没有变化。省略吐温80以及用吐温20替代它会导致细胞内K⁺减少。来自吐温40和吐温60培养物的细胞还明显含有更少的Na⁺。
