Ng W S, Ng M H, Inoue M, Tan Y H
Clin Exp Immunol. 1981 Jun;44(3):594-602.
We studied some of the requirements for production of immune interferon (IFN-gamma) by human peripheral leucocytes following stimulation with concanavalin A (Con A). Synthesis of IFN-gamma commenced 2 hr after the cells were exposed to the lectin and continued for about 20 hr thereafter. IFN-gamma production during the latter period was independent of free Con A in the culture medium but it was sustained by continued Con A binding and required active de novo protein synthesis. Egg lecithin, which inhibits the blastogenic response, did not affect IFN-gamma production.
我们研究了用伴刀豆球蛋白A（Con A）刺激后人外周血白细胞产生免疫干扰素（IFN-γ）的一些条件。细胞接触凝集素后2小时开始合成IFN-γ，并在此后持续约20小时。后期IFN-γ的产生与培养基中游离的Con A无关，但它通过Con A的持续结合得以维持，并且需要有活性的从头蛋白质合成。抑制母细胞形成反应的卵磷脂不影响IFN-γ的产生。
