Benyush V A, Luckash V G, Shtannikov A V
Hum Genet. 1977 Nov 10;39(2):169-75. doi: 10.1007/BF00287009.
A method of quantitative analysis of C segments in human chromosomes 1, 9, and 16 based on longitudinal densitometry has been developed. The way of fitting apparatus data to visual estimates is represented. Density curve parameters not dependent on stain intensity were used. The general C band length error is approx. 0.05 micrometer. Heteromorphic chromosome 16 pairs have been investigated with this method. A significant difference (about 0.18 micrometer) between C bands of the homologues has been detected in chromosome 16. It has been calculated that C bands can be distinquished if the mean difference between the length of the homologues is more than 15%.
已开发出一种基于纵向光密度测定法对人类1号、9号和16号染色体中C片段进行定量分析的方法。介绍了将仪器数据与视觉估计值拟合的方法。使用了不依赖于染色强度的密度曲线参数。C带总长度误差约为0.05微米。用该方法研究了异型16号染色体对。在16号染色体中检测到同源染色体的C带之间存在显著差异（约0.18微米）。经计算，如果同源染色体长度的平均差异超过15%，则可以区分C带。
