由17β-雌二醇-3-半琥珀酸酯-牛血清白蛋白产生的抗血清的特异性。
The specificity of antisera raised by oestradiol-17beta-3-hemisuccinyl-bovine serum albumin.
作者信息
Exley D, Woodhams B
出版信息
Steroids. 1976 Jun;27(6):813-20. doi: 10.1016/0039-128x(76)90140-9.
PMID:59968
Abstract
The preparation of pure oestradiol-17beta-3-hemisuccinyl-bovine serum albumin conjugate is described. Contrary to previous findings this antigen raised reasonably specific antisera in rabbits which possessed a cross reaction of only 2.0% with oestrone, and 0.8% with oestriol. The production of this specific antisera is considered to be due to the high purity of the antigen. The role of the C-3 phenolic hemisuccinyl linkage of the antigen in raising this specific antisera is discussed.
摘要
本文描述了纯β-雌二醇-3-半琥珀酸酯-牛血清白蛋白偶联物的制备方法。与之前的研究结果相反，这种抗原在兔子体内引发了相当特异的抗血清，该抗血清与雌酮的交叉反应仅为2.0%，与雌三醇的交叉反应为0.8%。这种特异性抗血清的产生被认为是由于抗原的高纯度。文中还讨论了抗原的C-3酚半琥珀酸酯键在引发这种特异性抗血清中的作用。
相似文献
1
The specificity of antisera raised by oestradiol-17beta-3-hemisuccinyl-bovine serum albumin.
Steroids. 1976 Jun;27(6):813-20. doi: 10.1016/0039-128x(76)90140-9.
2
The radioimmunoassay of steroid glucuronides. The oestrogen C-3 glucuronides as haptens.
Biochem J. 1975 Nov;151(2):369-76. doi: 10.1042/bj1510369.
3
Preparation of specific antiserum to estradiol 3-glucuronide.
J Steroid Biochem. 1982 Nov;17(5):511-5. doi: 10.1016/0022-4731(82)90009-7.
4
Specificity of antisera raised against estradiol 12-bovine serum albumin conjugates.
Chem Pharm Bull (Tokyo). 1985 Mar;33(3):1154-8. doi: 10.1248/cpb.33.1154.
5
Preparation of specific antisera to estradiol 17-glucuronide.
J Steroid Biochem. 1978 Aug;9(8):785-90. doi: 10.1016/0022-4731(78)90201-7.
6
Preparation of specific antisera to 15alpha-hydroxyestrogens.
Steroids. 1999 Aug;64(8):551-7. doi: 10.1016/s0039-128x(99)00031-8.
7
Antisera highly specific for 17 -oestradiol.
Steroids. 1971 Nov;18(5):605-20. doi: 10.1016/0039-128x(71)90073-0.
8
Radioimmunological methods for the estimation of oestrone, oestradiol-17beta and oestriol in pregnancy plasma.
Acta Obstet Gynecol Scand Suppl. 1974;32(0):5-19. doi: 10.3109/00016347409156389.
9
Production and specificity of antisera raised against 25-hydroxyvitamin D3-[C-3]-bovine serum albumin conjugates.
Steroids. 1992 Oct;57(10):488-93. doi: 10.1016/0039-128x(92)90043-9.
10
Characterization of antisera to 2-hydroxyestradiol and 4-hydroxyestradiol using 6-(O-carboxymethyl)oxime- and 17-hemisuccinate-bovine serum albumin conjugates and radioimmunoassay.
Steroids. 1985 Oct-Nov;46(4-5):857-65. doi: 10.1016/0039-128x(85)90034-0.
引用本文的文献
1
Rabbit anti-steroid antisera: a study of titers and specificities over a 22-week period.
Experientia. 1981 Nov 15;37(11):1203-4. doi: 10.1007/BF01989919.
Zotero 插件