Exley D, Woodhams B
Steroids. 1976 Jun;27(6):813-20. doi: 10.1016/0039-128x(76)90140-9.
The preparation of pure oestradiol-17beta-3-hemisuccinyl-bovine serum albumin conjugate is described. Contrary to previous findings this antigen raised reasonably specific antisera in rabbits which possessed a cross reaction of only 2.0% with oestrone, and 0.8% with oestriol. The production of this specific antisera is considered to be due to the high purity of the antigen. The role of the C-3 phenolic hemisuccinyl linkage of the antigen in raising this specific antisera is discussed.
本文描述了纯β-雌二醇-3-半琥珀酸酯-牛血清白蛋白偶联物的制备方法。与之前的研究结果相反，这种抗原在兔子体内引发了相当特异的抗血清，该抗血清与雌酮的交叉反应仅为2.0%，与雌三醇的交叉反应为0.8%。这种特异性抗血清的产生被认为是由于抗原的高纯度。文中还讨论了抗原的C-3酚半琥珀酸酯键在引发这种特异性抗血清中的作用。
