雌二醇3-葡萄糖醛酸苷特异性抗血清的制备。

Preparation of specific antiserum to estradiol 3-glucuronide.

作者信息

Shimada K, Ohkubo T, Tanaka M, Yoshida F, Nambara T

出版信息

J Steroid Biochem. 1982 Nov;17(5):511-5. doi: 10.1016/0022-4731(82)90009-7.

Abstract

The preparation and antigenic properties of estradiol 3-glucuronide-bovine serum albumin (BSA) conjugate in which the hapten is coupled to the carrier protein through the aminomethyl group at C-6 by glutaraldehyde, have been described. Antibody raised against the immunogen in the rabbit possessed high affinity (Ka = 3.7 x 10(9) M-1) and excellent specificity to estradiol 3-glucuronide, exhibiting no significant cross-reactivities with estrogen glucuronides except for estriol 3-glucuronide (3.64%) and no cross-reactions with free estrogens, their sulfates and other steroids.

摘要

已描述了雌二醇3-葡萄糖醛酸苷-牛血清白蛋白（BSA）偶联物的制备及其抗原特性，其中半抗原通过戊二醛与载体蛋白在C-6位的氨甲基相连。用该免疫原在兔体内产生的抗体对雌二醇3-葡萄糖醛酸苷具有高亲和力（Ka = 3.7 x 10⁹ M⁻¹）和优异的特异性，除了雌三醇3-葡萄糖醛酸苷（3.64%）外，与雌激素葡萄糖醛酸苷无明显交叉反应，与游离雌激素、其硫酸盐及其他类固醇无交叉反应。