Protective action of series of new mono-pyridinium salts and their effect on "aging" of acetylcholine esterase inhibited by fluostigmine.

The protection of acetylcholine esterase (AChE) and the influence on "aging" of AChE--inhibitor complex of several new methylpyridinium derivatives was studied in human blood cells and in mice poisoned with diisopropyl phosphorofluoridate (fluostigmine, DFP). A correlation between the chemical structure of the compounds and their biological activity has been found. The most potent was compound PAN-W-24, having 4-N(CH3)3 substitutent, which markedly prolonged the half-life of "aging" of the AChE-fluostigmine complex.