Kaufman M, Straisfeld C, Pinsky L
Somatic Cell Genet. 1977 Jan;3(1):17-25. doi: 10.1007/BF01550984.
Specific 5alpha-dihydrotestosterone (DHT) binding capacity (Bmax) has been determined for human skin fibroblast strains from non-genital areas of males and females (N = 8), as well as prepuce and labium majus (N = 9). Genital strains had a mean three times that of non-genital ones (32 vs. 11 fmol/mg cell protein). There were no sex differences. Variation among strains was not simply correlated with donor age; that within strains was unrelated to in vitro age. The lowest values for genital strains overlapped the nongenital ones; those of the nongenital strains approached the limit of detectability. These results parallel those for delta4-3-ketosteroid 5alpha-reductase activity. Thus, serially cultured genital and nongenital skin fibroblasts express their relative differentiative ancestry as androgen target cells. This expression may affect the diagnosis of androgen insensitivity and certain inborn errors of metabolism; its variability is discussed in terms of clonal heterogeneity.
已测定来自男性和女性非生殖器区域（N = 8）以及包皮和大阴唇（N = 9）的人皮肤成纤维细胞株的特异性5α - 双氢睾酮（DHT）结合能力（Bmax）。生殖器细胞株的平均值是非生殖器细胞株的三倍（32对11 fmol/mg细胞蛋白）。不存在性别差异。不同细胞株之间的差异与供体年龄并非简单相关；同一细胞株内的差异与体外培养时间无关。生殖器细胞株的最低值与非生殖器细胞株的最低值重叠；非生殖器细胞株的最低值接近可检测极限。这些结果与δ4 - 3 - 酮类固醇5α - 还原酶活性的结果相似。因此，连续培养的生殖器和非生殖器皮肤成纤维细胞作为雄激素靶细胞表达了它们相对的分化谱系。这种表达可能会影响雄激素不敏感和某些先天性代谢缺陷的诊断；根据克隆异质性对其变异性进行了讨论。
