Bauknecht T
Hum Genet. 1977 Dec 23;39(3):321-8. doi: 10.1007/BF00295427.
Cultured human fibroblasts and amniotic fluid cells (AF cells) were examined for the presence of steroid hormone receptors. In both cell types, the androgen (DHT) or glucocorticoid (dexamethasone) receptor was detected, but not the estrogen receptor. Binding parameters in fibroblasts were: for androgen: KD = 3.7 X 10(-9) M, Bmax = 13 fmol/mg; for dexamethasone: KD = 4.5 X 10(-9) M, Bmax = 120 fmol/mg. Binding parameters in AF cells were: for androgen: KD = 4 X 10(-9) M, Bmax = 8 fmol/mg; for dexamethasone: KD = 1.9 X 10(-8) M, Bmax = 375 fmol/mg. Cultured cells derived from the gonads (of a patient with 17beta-ketosteroid reductase deficiency) seem to have more receptors than cells from extragenital body parts (Bmax = 21 fmol). With the aid of gel chromatography, the molecular weight of the androgen receptor was estimated to be 30-40 000D.
检测培养的人成纤维细胞和羊水细胞（AF细胞）中是否存在甾体激素受体。在这两种细胞类型中，检测到了雄激素（双氢睾酮，DHT）或糖皮质激素（地塞米松）受体，但未检测到雌激素受体。成纤维细胞中的结合参数如下：对于雄激素：解离常数（KD）= 3.7×10⁻⁹ M，最大结合容量（Bmax）= 13 fmol/mg；对于地塞米松：KD = 4.5×10⁻⁹ M，Bmax = 120 fmol/mg。AF细胞中的结合参数如下：对于雄激素：KD = 4×10⁻⁹ M，Bmax = 8 fmol/mg；对于地塞米松：KD = 1.9×10⁻⁸ M，Bmax = 375 fmol/mg。来自性腺（一名患有17β-酮类固醇还原酶缺乏症患者的性腺）的培养细胞似乎比来自生殖器外身体部位的细胞具有更多的受体（Bmax = 21 fmol）。借助凝胶色谱法，估计雄激素受体的分子量为30 - 40 000D。
