Rothberg S, Axilrod G D
Science. 1967 Apr 7;156(3771):90-3. doi: 10.1126/science.156.3771.90.
Insoluble epidermal proteins (possibly keratin), previously considered inert to enzyme action, were solubilized by either trypsin or chymotrypsin. Cleavage of the disulfide bonds prior to enzymatic action is not necessary. In addition, the enzymatic action on intact epidermis is not influenced by the presence or absence of endogenous lipids, soluble proteins, peptides, or amino acids. Solubilization of epidermal protein by chymotrypsin is inhibited by the supernatant solution of the homogenized epidermis.
不溶性表皮蛋白（可能是角蛋白），以前被认为对酶的作用呈惰性，可被胰蛋白酶或胰凝乳蛋白酶溶解。在酶作用之前切断二硫键并非必要。此外，对完整表皮的酶作用不受内源性脂质、可溶性蛋白质、肽或氨基酸的存在与否的影响。胰凝乳蛋白酶对表皮蛋白的溶解作用受到表皮匀浆上清液的抑制。
