Park C E, Berger L R
J Bacteriol. 1967 Jan;93(1):230-6. doi: 10.1128/jb.93.1.230-236.1967.
Cells of Rhodomicrobium vannielii grown at 29 C in a lactate-containing medium were extracted at room temperature with organic solvents. The extractable fraction contained the bulk of the simple lipid (1.87% of cell dry weight) and complex lipids (phospholipids, 4.2%; sulfolipid, 0.01%), coenzyme Q (0.09%), and pigments (carotenoids 1.2%; bacteriochlorophyll, 1.9%). The cell residue contained the bound lipids (nonpolar fatty acid fraction, 1.86%; polar hydroxy fatty acids, 0.49%). The residue also contained poly-beta-hydroxybutyric acid (0.2%), which was extracted in boiling chloroform. In both the simple and complex lipids, vaccenic acid (11-octadecenoic acid) was the largest single component (approximately 90% in each fraction). The fatty acids of the bound lipid contained 35% vaccenic acid, even- and odd-numbered saturated and unsaturated straight-chain fatty acids, cyclopropane-, branched-, and alpha- and beta-hydroxy fatty acids. The extractable lipids contained only straight-chain saturated and unsaturated even-numbered fatty acids. Nearly 60% of hydroxy fatty acid fraction was alpha-hydroxydodecanoic acid (24%) and beta-hydroxydodecanoic acid (34.5%). Coenzyme Q was crystallized and identified as Q(9) on the basis of melting point and chromatographic properties. Q(10) had been previously reported.
在含乳酸培养基中于29℃培养的万氏红微菌细胞，在室温下用有机溶剂进行提取。可提取部分含有大部分的简单脂质（占细胞干重的1.87%）和复合脂质（磷脂，4.2%；硫脂，0.01%）、辅酶Q（0.09%）以及色素（类胡萝卜素1.2%；细菌叶绿素，1.9%）。细胞残渣含有结合脂质（非极性脂肪酸部分，1.86%；极性羟基脂肪酸，0.49%）。残渣中还含有聚-β-羟基丁酸（0.2%），其在沸腾的氯仿中被提取出来。在简单脂质和复合脂质中，反式vaccenic酸（11-十八碳烯酸）都是最大的单一成分（每个部分中约占90%）。结合脂质的脂肪酸含有35%的vaccenic酸、偶数和奇数的饱和与不饱和直链脂肪酸、环丙烷脂肪酸、支链脂肪酸以及α-和β-羟基脂肪酸。可提取脂质仅含有直链饱和和不饱和偶数脂肪酸。羟基脂肪酸部分近60%是α-羟基十二烷酸（24%）和β-羟基十二烷酸（34.5%）。辅酶Q被结晶，并根据熔点和色谱性质鉴定为Q(9)。此前曾报道过Q(10)。
