Johnson R C, Harris V G
J Bacteriol. 1967 Jul;94(1):27-31. doi: 10.1128/jb.94.1.27-31.1967.
The minimal growth temperature of the pathogenic leptospires is between 13 and 15 C. The saprophytic leptospires have a minimal growth temperature between 5 and 10 C, or approximately 5 C below that of the pathogens. The capability of the saprophytic leptospires to grow at temperatures below those which allow the growth of the pathogenic leptospires provides a simple method of discrimination. With an inoculum yielding approximately 8 x 10(7) cells per ml in the test medium and an incubation temperature of 13 C, the saprophytic leptospires were easily differentiated from the pathogenic leptospires. All 13 saprophytic leptospires tested grew in the 10% rabbit serum medium at 13 C, whereas none of the 20 pathogens grew during the 30-day incubation period.
致病性钩端螺旋体的最低生长温度在13至15摄氏度之间。腐生钩端螺旋体的最低生长温度在5至10摄氏度之间，或比病原体的最低生长温度低约5摄氏度。腐生钩端螺旋体在低于致病性钩端螺旋体生长温度的条件下生长的能力提供了一种简单的鉴别方法。在测试培养基中接种量约为每毫升8×10⁷个细胞，培养温度为13摄氏度时，腐生钩端螺旋体很容易与致病性钩端螺旋体区分开来。所有测试的13株腐生钩端螺旋体在13摄氏度的10%兔血清培养基中生长，而20株病原体在30天的培养期内均未生长。
