艾氏腹水癌细胞表面膜中唾液酸的连接
The linkage of sialic acid in the Ehrlich ascites-carcinoma cell surface membrane.
作者信息
Langley O K, Ambrose E J
出版信息
Biochem J. 1967 Jan;102(1):367-72. doi: 10.1042/bj1020367.
Abstract
- The sialic acid content of fresh and fixed Ehrlich ascites cells was determined by incubation with neuraminidase and analysis of the supernatants. 2. The content of sialic acid was also determined on ultrasonically disrupted cells either with or without prior neuraminidase treatment, and the location of sialic acid in the cell is discussed. 3. The sialic acids, cleaved from cells by neuraminidase, were identified chromatographically. 4. Proteolytic enzymes were used to isolate from cells a mucopeptide containing sialic acid and galactosamine in almost equimolar proportions. 5. The nature of the carbohydrate-amino acid bond in the muco-peptide was investigated by alkaline hydrolysis. 6. A suggestion is made about the particular amino acids involved in the sugar-peptide bond.
摘要
- 通过与神经氨酸酶孵育并分析上清液来测定新鲜和固定的艾氏腹水细胞的唾液酸含量。2. 还对经或未经神经氨酸酶预处理的超声破碎细胞进行了唾液酸含量测定，并讨论了唾液酸在细胞中的定位。3. 对经神经氨酸酶从细胞中裂解出来的唾液酸进行了色谱鉴定。4. 使用蛋白水解酶从细胞中分离出一种含有几乎等摩尔比例的唾液酸和半乳糖胺的粘肽。5. 通过碱性水解研究了粘肽中碳水化合物 - 氨基酸键的性质。6. 对糖肽键中涉及的特定氨基酸提出了一种推测。
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