一项关于抗体和酶处理后通过细胞电泳对肿瘤细胞、胎儿细胞和淋巴结细胞表面的研究。
A study of the cell surface of tumour, foetal and lymph-node cells by cell electrophoresis after antibody and enzymic treatment.
作者信息
Woo J, Cater D B
出版信息
Biochem J. 1972 Aug;128(5):1273-84. doi: 10.1042/bj1281273.
Abstract
- Rabbit anti-(rat foetal liver) serum, absorbed with adult rat liver cells, decreased the electrophoretic mobility of foetal liver cells by 51% and rat hepatoma cells by 45%, indicating the presence of a foetal-type antigen on the hepatoma cell membrane. 2. The chemical nature of the surface antigen was investigated. Incubation with neuraminidase had no effect on adult liver cells but decreased the electrophoretic mobility of foetal liver cells by 51% and of hepatoma cells by 34%; the effect of antiserum was decreased to one-fifth. 3. Sialic acid, or the supernatant from neuraminidase-treated cells, partially blocked the decrease in electrophoretic mobility induced by antiserum. 4. The pH-electrophoretic mobility curves of hepatoma cells treated with antisera were consistent with a sialic acidcontaining antigen on the surface of the tumour cells. 5. Treatment with ribonuclease did not decrease the electrophoretic mobility of adult-liver cells, but decreased that of the foetal liver cells by 17% and hepatoma cells by 29%. 6. In parallel studies made with mouse BP8 ascites-tumour cells ribonuclease decreased the electrophoretic mobility by 39%, that of normal mouse lymph-node cells by 4.8% and allergized mouse lymph-node cells by 13.3%. 7. Trypsin treatment also decreased the electrophoretic mobility of hepatoma cells by 22%.
摘要
- 用成年大鼠肝细胞吸收的兔抗（大鼠胎儿肝脏）血清，使胎儿肝细胞的电泳迁移率降低了51%，大鼠肝癌细胞的电泳迁移率降低了45%，这表明肝癌细胞膜上存在胎儿型抗原。2. 对表面抗原的化学性质进行了研究。用神经氨酸酶孵育对成年肝细胞没有影响，但使胎儿肝细胞的电泳迁移率降低了51%，肝癌细胞的电泳迁移率降低了34%；抗血清的作用降低到了五分之一。3. 唾液酸或经神经氨酸酶处理的细胞的上清液部分阻断了抗血清诱导的电泳迁移率降低。4. 用抗血清处理的肝癌细胞的pH-电泳迁移率曲线与肿瘤细胞表面含唾液酸的抗原一致。5. 用核糖核酸酶处理不会降低成年肝细胞的电泳迁移率，但会使胎儿肝细胞的电泳迁移率降低17%，肝癌细胞的电泳迁移率降低29%。6. 在对小鼠BP8腹水肿瘤细胞进行的平行研究中，核糖核酸酶使电泳迁移率降低了39%，正常小鼠淋巴结细胞的电泳迁移率降低了4.8%，致敏小鼠淋巴结细胞的电泳迁移率降低了13.3%。7. 胰蛋白酶处理也使肝癌细胞的电泳迁移率降低了22%。
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