Department of Physiology and Biophysics, University of Washington, School of Medicine, Box 357290, Seattle, WA, 98195-7290, USA.
Prog Biophys Mol Biol. 2022 Mar-May;169-170:12-20. doi: 10.1016/j.pbiomolbio.2021.11.003. Epub 2021 Nov 29.
This retrospective traces the hypothesis of ion channels from an early statement in a 1970 essay in this journal (Hille, B., 1970, Prog. Biophys. Mol. Biol. 21, 1-32) to its realization today in biophysical, molecular, biochemical, and structural terms. The Na and K channels of the action potential have been isolated, reconstituted, cloned, mutated, and expressed. They are conformationally flexible, multi-pass glycosylated membrane proteins. Refined atomic structures of several conformational states are known. The discoveries over this half century history illustrate the growth of a field from initial ideas to a mature discipline of biology, physiology, and biomedical science.
这篇回顾追溯了离子通道假说的发展历程，从该杂志上一篇 1970 年的论文中的早期论述（Hille，B.，1970 年，Prog. Biophys. Mol. Biol. 21，1-32）到今天在生物物理、分子、生化和结构方面的实现。动作电位的钠和钾通道已经被分离、重建、克隆、突变和表达。它们是构象灵活的、多跨糖基化的膜蛋白。几个构象状态的精细原子结构已经为人所知。这半个世纪的发现说明了一个领域从最初的想法到生物学、生理学和生物医学科学的成熟学科的发展过程。
