Schafer I A, Silverman L, Sullivan J C, Robertson W V
J Cell Biol. 1967 Jul;34(1):83-95. doi: 10.1083/jcb.34.1.83.
Fibroblasts grown in medium containing less than 1 microg of ascorbic acid per milliliter showed evidence of ascorbic acid deficiency when compared with cells grown in medium containing 50 microg of ascorbic acid per milliliter. This was manifested morphologically by dilated endoplasmic reticulum, a decrease in number, size, and intensity of staining of the mitochondria, by defective intercellular fibril formation, and by easy disaggregation of the cells from the intercellular matrix after treatment with pronase. When 50 microg per milliliter of ascorbic acid was incorporated into the medium, the altered morphology was corrected, banded fibrils were produced which were organized into bundles, and the cells were tightly bound in a matrix which was resistant to disaggregation with a variety of proteolytic enzymes. Collagen and sulfated glycosaminoglycan synthesis were less in the control than in the ascorbic acid supplemented cells. Similar morphological and chemical changes have been reported in the connective tissue of scorbutic animals. The effects of low ascorbic acid concentration on fibroblasts in culture indicate that these cells require ascorbic acid to maintain connective tissue functions.
与在每毫升含50微克抗坏血酸的培养基中生长的细胞相比，在每毫升含少于1微克抗坏血酸的培养基中生长的成纤维细胞显示出抗坏血酸缺乏的迹象。这在形态学上表现为内质网扩张、线粒体数量、大小和染色强度降低、细胞间原纤维形成缺陷以及用链霉蛋白酶处理后细胞易于从细胞间基质中解离。当每毫升培养基中加入50微克抗坏血酸时，形态改变得到纠正，产生了带状原纤维并组织成束，细胞紧密结合在对多种蛋白水解酶的解离具有抗性的基质中。对照中的胶原蛋白和硫酸化糖胺聚糖合成比补充抗坏血酸的细胞中的少。在坏血病动物的结缔组织中也报道了类似的形态和化学变化。低抗坏血酸浓度对培养的成纤维细胞的影响表明，这些细胞需要抗坏血酸来维持结缔组织功能。
