对数变换在服从米氏动力学的系统的传输参数计算中的应用。
The use of the logarithmic transformation in the calculation of the transport parameters of a system that obeys Michaelis-Menten kinetics.
作者信息
Barber H E, Welch B L, Mackay D
出版信息
Biochem J. 1967 Apr;103(1):251-5. doi: 10.1042/bj1030251.
Abstract
- A logarithmic method is described for the calculation of the transport parameters, K(m) and V(max.)' of a biological system obeying Michaelis-Menten kinetics. 2. This logarithmic method leads to a way of estimating the transport parameters that has not apparently been used previously. It allows the separation of variance due to V(max.) from other variance, and so reduces the fiducial limits that can be placed on an estimation of K(m). 3. The results of studies on the transport of l-histidine and l-monoiodohistidine by rat intestinal sacs in vitro have been used to illustrate the application of the new method. Estimates of the transport parameters have also been made by two alternative procedures. The relative merits of the three methods are discussed.
摘要
- 描述了一种对数方法，用于计算遵循米氏动力学的生物系统的转运参数K(m)和V(max.)。2. 这种对数方法引出了一种估计转运参数的方法，该方法此前显然未被使用过。它允许将因V(max.)产生的方差与其他方差分离，从而缩小了对K(m)估计的置信限。3. 对大鼠离体肠囊转运L-组氨酸和L-单碘组氨酸的研究结果已用于说明新方法的应用。还通过另外两种方法对转运参数进行了估计。讨论了这三种方法的相对优点。
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