Steinberg V I, Hart E J, Handley J, Goldberg I D
J Clin Microbiol. 1976 Jul;4(1):87-91. doi: 10.1128/jcm.4.1.87-91.1976.
Six isolates from normal throat samples have been shown to contain phage active against Neisseria perflava. The phage isolates were similar in terms of host range, latent period, burst size, antigenic properties, morphology, and nucleic acid content. Neutralization studies with antisera demonstrated that the isolates exhibited a very high degree of serological relatedness. These results taken together suggested that the isolates represented a single strain of bacteriophage. This phage, which we have designated NP-1, exhibited a high degree of host specificity, attacking only one of the several strains of N. perflava tested and none of the other species tested. One-step growth experiments yielded minimum latent periods of approximately 35 min; average burst sizes varied from 34 to 63 plaque-forming units per cell. Electron micrographs revealed particles with heads averaging 75 nm in diameter and tails averaging 300 nm in length and 18 nm in diameter. The phage contained double-stranded DNA with a guanine plus cytosine content of 38%.
已证明从正常咽喉样本中分离出的6株菌株含有对全黄奈瑟菌有活性的噬菌体。这些噬菌体分离株在宿主范围、潜伏期、裂解量、抗原特性、形态和核酸含量方面相似。用抗血清进行的中和研究表明，这些分离株表现出高度的血清学相关性。综合这些结果表明，这些分离株代表单一的噬菌体菌株。我们将这种噬菌体命名为NP-1，它表现出高度的宿主特异性，仅攻击所测试的几种全黄奈瑟菌菌株中的一种，而不攻击所测试的其他物种。一步生长实验产生的最短潜伏期约为35分钟；平均裂解量为每个细胞产生34至63个噬菌斑形成单位。电子显微镜照片显示，这些颗粒的头部平均直径为75纳米，尾部平均长度为300纳米，直径为18纳米。该噬菌体含有双链DNA，鸟嘌呤加胞嘧啶含量为38%。
