Weiss B D, Capage M A, Kessel M, Benson S A
Department of Microbiology, University of Maryland, College Park 20742.
J Bacteriol. 1994 Jun;176(11):3354-9. doi: 10.1128/jb.176.11.3354-3359.1994.
We have isolated and characterized a lytic double-stranded DNA Xanthomonas campestris pv. campestris bacteriophage (XTP1) capable of mediating generalized transduction. The phage transduces chromosomal markers at frequencies of 10(-5) to 10(-6) transductants per PFU. We demonstrated its genetic utility by the isolation and cotransduction of linked transposon insertions to a nonselectable locus, xgl, required for the cleavage of 5-bromo-3-chloro-indoyl-beta-D-galactoside and showed that rif and str alleles in X. campestris are 75% linked. One-step growth experiments showed that the latent and rise periods were each 2 h and the average burst size was 35. The DNA genome is approximately 180 kb, presumably modified in a sequence-specific manner, and may be covalently attached to protein(s). Electron micrographs show the phage particle to have an icosahedral head and contractile tail with tail fibers uniquely attached to a location 40 nm proximal from the end of the tail.
我们分离并鉴定了一种能够介导普遍性转导的溶菌性双链DNA野油菜黄单胞菌野油菜致病变种噬菌体（XTP1）。该噬菌体以每噬菌斑形成单位（PFU）产生10^(-5)至10^(-6)个转导子的频率转导染色体标记。我们通过将连锁转座子插入片段分离并共转导至一个不可选择的位点xgl（5-溴-3-氯吲哚-β-D-半乳糖苷裂解所必需的位点），证明了其遗传效用，并表明野油菜黄单胞菌中的rif和str等位基因有75%的连锁性。一步生长实验表明，潜伏期和上升期均为2小时，平均裂解量为35。DNA基因组约为180 kb，推测是以序列特异性方式修饰的，并且可能与蛋白质共价连接。电子显微镜照片显示，噬菌体颗粒具有二十面体头部和收缩性尾部，尾丝独特地附着在距尾部末端40 nm处的一个位置。
